##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tru9I       Tsp45I      TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI           94  GCGG                 CCGC(-3/-1)
AcsI           77  GAATTC               [GA]^AATT[CT]
AfaI          114  GTAC                 GT^AC
AluI           50  AGCT                 AG^CT
AocI          160  CCTGAGG              CC^T[ACGT]AGG
ApoI           77  GAATTC               [GA]^AATT[CT]
Asp700I       171  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          24  GGACC                G^G[AT]CC
BbvI           48  GCAGC                GCAGC(8/12)
BsaAI         115  TACGTG               [CT]AC^GT[GA]
BsaJI           5  CCCAGG               C^C[ACGT][ACGT]GG
BssKI           6  CCAGG                ^CC[ACGT]GG
BstF5I        156  CATCC                GGATG(2/0)
BstNI           6  CCAGG                CC^[AT]GG
BstYI           9  GGATCT               [GA]^GATC[CT]
Bsu36I        160  CCTGAGG              CC^T[ACGT]AGG
DraII          23  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eco81I        160  CCTGAGG              CC^T[ACGT]AGG
EcoO109I       23  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI          77  GAATTC               G^AATTC
EcoRII          6  CCAGG                ^CC[AT]GG
FauI           94  GCGGG                CCCGC(4/6)
Fnu4HI         48  GCAGC                GC^[ACGT]GC
FokI          156  CATCC                GGATG(9/13)
HinfI         171  GAATC                G^A[ACGT]TC
ItaI           48  GCAGC                GC^[ACGT]GC
MaeII         116  ACGT                 A^CGT
MflI            9  GGATCT               [GA]^GATC[CT]
MspA1I         49  CAGCTG               C[AC]G^C[GT]G
MvaI            6  CCAGG                CC^[AT]GG
PpuMI          23  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          49  CAGCTG               CAG^CTG
RsaI          114  GTAC                 GT^AC
ScrFI           6  CCAGG                CC^[ACGT]GG
SfaNI         155  GCATC                GCATC(5/9)
TfiI          171  GAATC                G^A[AT]TC
TseI           48  GCAGC                G^C[AT]GC
Tsp509I        78  AATT                 ^AATT
XhoII           9  GGATCT               [GA]^GATC[CT]
XmnI          171  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwI          139  GATCC                GGATC(4/5)
                9  GGATC               
DdeI           13  CTCAG                C^T[ACGT]AG
              161  CTGAG               
DpnII          10  GATC                 ^GATC
              139  GATC                
HaeIII          3  GGCC                 GG^CC
              123  GGCC                
MboI           10  GATC                 ^GATC
              139  GATC                
MboII          33  GAAGA                GAAGA(8/7)
               54  GAAGA               
NdeII          10  GATC                 ^GATC
              139  GATC                
Sau3AI         10  GATC                 ^GATC
              139  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

Cfr13I      MnlI        Sau96I