Restriction map for
pFN21AE1840
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
CelII ClaI CpoI DpnII DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NarI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 625 ACGTGT A^C[GA][CT]GT
AspHI 912 GTGCTC G[AT]GC[AT]^C
BfaI 775 CTAG C^TAG
BglI 302 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 226 GAGACC GGTCTC(1/5)
BseRI 1045 GAGGAG GAGGAG(10/8)
BsiHKAI 912 GTGCTC G[AT]GC[AT]^C
BsmBI 697 GAGACG CGTCTC(1/5)
BsrDI 1032 GCAATG GCAATG(2/0)
BstF5I 237 GGATG GGATG(2/0)
BstXI 812 CCAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 727 GACTCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 636 CCATGG C^C[GA][CT]GG
EaeI 26 TGGCCG [CT]^GGCC[GA]
EarI 606 GAAGAG CTCTTC(1/4)
FokI 237 GGATG GGATG(9/13)
Ksp632I 606 GAAGAG CTCTTC(1/4)
MaeI 775 CTAG C^TAG
MboII 606 GAAGA GAAGA(8/7)
MspA1I 927 CAGCTG C[AC]G^C[GT]G
NcoI 636 CCATGG C^CATGG
PvuII 927 CAGCTG CAG^CTG
ScaI 884 AGTACT AGT^ACT
SexAI 839 ACCTGGT A^CC[AT]GGT
XcmI 755 CCAATGAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 15 CGCG CG^CG
360 CGCG
AcyI 99 GACGCC G[GA]^CG[CT]C
571 GACGCC
AlwNI 706 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
864 CAGCTTCTG
AocI 231 CCTGAGG CC^T[ACGT]AGG
615 CCTCAGG
ApaI 146 GGGCCC GGGCC^C
785 GGGCCC
AvaI 395 CCCGGG C^[CT]CG[GA]G
917 CCCGGG
AviII 243 TGCGCA TGC^GCA
662 TGCGCA
BbrPI 586 CACGTG CAC^GTG
624 CACGTG
BpmI 304 CTGGAG CTGGAG(16/14)
451 CTGGAG
BsaHI 99 GACGCC G[GA]^CG[CT]C
571 GACGCC
BsmFI 836 GGGAC GGGAC(10/14)
180 GTCCC
BsoBI 395 CCCGGG C^[CT]CG[GA]G
917 CCCGGG
Bsp1407I 845 TGTACA T^GTACA
1055 TGTACA
BsrFI 458 GCCGGC [GA]^CCGG[CT]
872 GCCGGC
BsrGI 845 TGTACA T^GTACA
1055 TGTACA
BsrI 267 CCAGT ACTGG(1/-1)
746 CCAGT
BstAPI 935 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1032 GCAATGTGTGC
BstUI 15 CGCG CG^CG
360 CGCG
Bsu36I 231 CCTGAGG CC^T[ACGT]AGG
615 CCTCAGG
Cfr10I 458 GCCGGC [GA]^CCGG[CT]
872 GCCGGC
Eco81I 231 CCTGAGG CC^T[ACGT]AGG
615 CCTCAGG
FspI 243 TGCGCA TGC^GCA
662 TGCGCA
HgaI 99 GACGC GACGC(5/10)
571 GACGC
Hin1I 99 GACGCC G[GA]^CG[CT]C
571 GACGCC
MaeIII 164 GTGAC ^GT[ACGT]AC
488 GTGAC
MvnI 15 CGCG CG^CG
360 CGCG
NaeI 458 GCCGGC GCC^GGC
872 GCCGGC
NgoMIV 458 GCCGGC G^CCGGC
872 GCCGGC
PflMI 384 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
826 CCAAGGTCTGG
PmaCI 586 CACGTG CAC^GTG
624 CACGTG
PmlI 586 CACGTG CAC^GTG
624 CACGTG
PpuMI 551 AGGACCT [GA]G^G[AT]CC[CT]
836 GGGACCT
PstI 402 CTGCAG CTGCA^G
930 CTGCAG
SfaNI 238 GATGC GCATC(5/9)
593 GCATC
SmaI 395 CCCGGG CCC^GGG
917 CCCGGG
SspBI 845 TGTACA T^GTACA
1055 TGTACA
Tsp45I 164 GTGAC ^GT[GC]AC
488 GTGAC
Van91I 384 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
826 CCAAGGTCTGG
XmaCI 395 CCCGGG C^CCGGG
917 CCCGGG
XmaI 395 CCCGGG C^CCGGG
917 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI Aor51HI AvaII
BanII BbvI BcnI BmyI BsaAI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfcI SmlI StuI StyI
TseI TspRI