Restriction map for
pFN21AE5318
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BlnI BpuAI
BsaAI BsaHI BsaI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI MaeII MfeI
MluI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacI SalI SapI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 853 GGTACC G^GTACC
AccIII 776 TCCGGA T^CCGGA
AfaI 854 GTAC GT^AC
Alw44I 717 GTGCAC G^TGCAC
AlwI 488 GGATC GGATC(4/5)
ApaLI 717 GTGCAC G^TGCAC
Asp700I 570 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 853 GGTACC G^GTACC
AspHI 717 GTGCAC G[AT]GC[AT]^C
BalI 5 TGGCCA TGG^CCA
BcgI 823 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 692 CTAG C^TAG
BglII 168 AGATCT A^GATCT
BsaBI 163 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 776 TCCGGA [AT]^CCGG[AT]
BseAI 776 TCCGGA T^CCGGA
BsiHKAI 717 GTGCAC G[AT]GC[AT]^C
BspEI 776 TCCGGA T^CCGGA
BspHI 108 TCATGA T^CATGA
BsrFI 721 ACCGGC [GA]^CCGG[CT]
BstAPI 702 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 721 ACCGGC [GA]^CCGG[CT]
CpoI 778 CGGACCG CG^G[AT]CCG
EarI 385 GAAGAG CTCTTC(1/4)
EcoNI 51 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 483 CCATGG C^C[AT][AT]GG
HgaI 105 GCGTC GACGC(5/10)
KpnI 853 GGTACC GGTAC^C
Ksp632I 385 GAAGAG CTCTTC(1/4)
KspI 139 CCGCGG CCGC^GG
MaeI 692 CTAG C^TAG
MaeIII 39 GTCAC ^GT[ACGT]AC
MamI 163 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5 TGGCCA TGG^CCA
MroI 776 TCCGGA T^CCGGA
MscI 5 TGGCCA TGG^CCA
NcoI 483 CCATGG C^CATGG
PflMI 405 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 452 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 706 CAGCTG CAG^CTG
RcaI 108 TCATGA T^CATGA
RsaI 854 GTAC GT^AC
RsrII 778 CGGACCG CG^G[AT]CCG
SacII 139 CCGCGG CCGC^GG
SbfI 612 CCTGCAGG CCTGCA^GG
Sse8387I 612 CCTGCAGG CCTGCA^GG
StyI 483 CCATGG C^C[AT][AT]GG
TaqI 286 TCGA T^CGA
Tsp45I 39 GTCAC ^GT[GC]AC
TthHB8I 286 TCGA T^CGA
Van91I 405 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 570 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 140 CGCG CG^CG
382 CGCG
AvaI 78 CCCGGG C^[CT]CG[GA]G
374 CCCGGG
BanI 73 GGTGCC G^G[CT][GA]CC
853 GGTACC
BglI 227 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
611 GCCTGCAGGGC
BlpI 239 GCTCAGC GC^T[ACGT]AGC
321 GCTGAGC
Bpu1102I 239 GCTCAGC GC^T[ACGT]AGC
321 GCTGAGC
BsoBI 78 CCCGGG C^[CT]CG[GA]G
374 CCCGGG
BstUI 140 CGCG CG^CG
382 CGCG
BstYI 168 AGATCT [GA]^GATC[CT]
488 GGATCT
CelII 239 GCTCAGC GC^T[ACGT]AGC
321 GCTGAGC
CfoI 291 GCGC GCG^C
550 GCGC
DpnII 169 GATC ^GATC
489 GATC
DraII 367 AGGGCCT [GA]G^G[ACGT]CC[CT]
452 GGGACCC
EcoO109I 367 AGGGCCT [GA]G^G[ACGT]CC[CT]
452 GGGACCC
FauI 345 GCGGG CCCGC(4/6)
540 GCGGG
HhaI 291 GCGC GCG^C
550 GCGC
HinP1I 291 GCGC G^CGC
550 GCGC
HphI 556 GGTGA GGTGA(8/7)
40 TCACC
MboI 169 GATC ^GATC
489 GATC
MflI 168 AGATCT [GA]^GATC[CT]
488 GGATCT
MvnI 140 CGCG CG^CG
382 CGCG
NdeII 169 GATC ^GATC
489 GATC
NlaIII 109 CATG CATG^
484 CATG
Sau3AI 169 GATC ^GATC
489 GATC
SmaI 78 CCCGGG CCC^GGG
374 CCCGGG
XhoII 168 AGATCT [GA]^GATC[CT]
488 GGATCT
XmaCI 78 CCCGGG C^CCGGG
374 CCCGGG
XmaI 78 CCCGGG C^CCGGG
374 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DsaI EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIV PleI PstI Sau96I ScrFI
SfcI TseI TspRI