Restriction map for
pFN21AE5742
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbeI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BstBI
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 754 AAATTT [GA]^AATT[CT]
Alw44I 169 GTGCAC G^TGCAC
ApaI 1196 GGGCCC GGGCC^C
ApaLI 169 GTGCAC G^TGCAC
ApoI 754 AAATTT [GA]^AATT[CT]
BglI 1015 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 922 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1498 GAGACG CGTCTC(1/5)
BspHI 434 TCATGA T^CATGA
BsrFI 974 GCCGGC [GA]^CCGG[CT]
BssSI 1311 CACGAG CACGAG(-5/-1)
Bst1107I 1432 GTATAC GTA^TAC
BstEII 177 GGTGACC G^GT[ACGT]ACC
BstPI 177 GGTGACC G^GT[ACGT]ACC
BstXI 1310 CCACGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1432 GTATAC GTA^TAC
Cfr10I 974 GCCGGC [GA]^CCGG[CT]
EarI 193 GAAGAG CTCTTC(1/4)
EcoNI 1704 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 177 GGTGACC G^GT[ACGT]ACC
HincII 160 GTCAAC GT[CT]^[GA]AC
HindII 160 GTCAAC GT[CT]^[GA]AC
HinfI 1041 GAATC G^A[ACGT]TC
Ksp632I 193 GAAGAG CTCTTC(1/4)
MamI 922 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1436 ACGCGT A^CGCGT
MseI 455 TTAA T^TAA
NaeI 974 GCCGGC GCC^GGC
NgoMIV 974 GCCGGC G^CCGGC
RcaI 434 TCATGA T^CATGA
SacI 361 GAGCTC GAGCT^C
SexAI 173 ACCTGGT A^CC[AT]GGT
TfiI 1041 GAATC G^A[AT]TC
Tru9I 455 TTAA T^TAA
Tsp509I 755 AATT ^AATT
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 421 GTCTAC GT^[AC][GT]AC
1432 GTATAC
AflIII 578 ACACGT A^C[GA][CT]GT
1436 ACGCGT
AocI 849 CCTGAGG CC^T[ACGT]AGG
1927 CCTCAGG
AspI 415 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
883 GACACCGTC
BclI 29 TGATCA T^GATCA
1481 TGATCA
BfaI 124 CTAG C^TAG
448 CTAG
BsaI 1921 GAGACC GGTCTC(1/5)
493 GGTCTC
BsrDI 1345 CATTGC GCAATG(2/0)
1349 GCAATG
Bsu36I 849 CCTGAGG CC^T[ACGT]AGG
1927 CCTCAGG
DraII 520 GGGGCCT [GA]G^G[ACGT]CC[CT]
1172 AGGGCCT
DraIII 172 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
579 CACGTGGTG
Eco81I 849 CCTGAGG CC^T[ACGT]AGG
1927 CCTCAGG
EcoO109I 520 GGGGCCT [GA]G^G[ACGT]CC[CT]
1172 AGGGCCT
FbaI 29 TGATCA T^GATCA
1481 TGATCA
MaeI 124 CTAG C^TAG
448 CTAG
NcoI 1391 CCATGG C^CATGG
1692 CCATGG
NspI 206 GCATGC [GA]CATG^[CT]
1910 GCATGC
PflFI 415 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
883 GACACCGTC
PvuII 616 CAGCTG CAG^CTG
1830 CAGCTG
SmaI 92 CCCGGG CCC^GGG
1415 CCCGGG
SphI 206 GCATGC GCATG^C
1910 GCATGC
Tth111I 415 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
883 GACACCGTC
XmaCI 92 CCCGGG C^CCGGG
1415 CCCGGG
XmaI 92 CCCGGG C^CCGGG
1415 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PmaCI
PmlI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII