Restriction map for
pFN21AB5945
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AlwI AlwNI
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbrPI BbvCI BcgI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRI FseI FspI HindIII HpaI
KpnI KspI MaeI MaeII MfeI
MflI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 1372 CGCG CG^CG
AcsI 817 AAATTC [GA]^AATT[CT]
AcyI 946 GGCGCC G[GA]^CG[CT]C
AhdI 308 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 665 AGCGCT AGC^GCT
ApaI 1229 GGGCCC GGGCC^C
ApoI 817 AAATTC [GA]^AATT[CT]
AspEI 308 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1309 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1146 TGGCCA TGG^CCA
BbeI 946 GGCGCC GGCGC^C
BbsI 151 GAAGAC GAAGAC(2/6)
BciVI 1023 GTATCC GTATCC(6/5)
BclI 1322 TGATCA T^GATCA
BpuAI 151 GAAGAC GAAGAC(2/6)
BsaBI 1348 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 946 GGCGCC G[GA]^CG[CT]C
BsmBI 1487 CGTCTC CGTCTC(1/5)
BsmFI 701 GGGAC GGGAC(10/14)
BspHI 102 TCATGA T^CATGA
BsrBI 1266 CCGCTC CCGCTC(-3/-3)
BstEII 475 GGTAACC G^GT[ACGT]ACC
BstPI 475 GGTAACC G^GT[ACGT]ACC
BstUI 1372 CGCG CG^CG
DraI 213 TTTAAA TTT^AAA
EaeI 1146 TGGCCA [CT]^GGCC[GA]
Eam1105I 308 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 665 AGCGCT AGC^GCT
EcoO65I 475 GGTAACC G^GT[ACGT]ACC
EcoRV 1457 GATATC GAT^ATC
EcoT22I 542 ATGCAT ATGCA^T
FbaI 1322 TGATCA T^GATCA
HapII 1041 CCGG C^CGG
HgaI 1373 GCGTC GACGC(5/10)
Hin1I 946 GGCGCC G[GA]^CG[CT]C
HincII 850 GTCAAC GT[CT]^[GA]AC
HindII 850 GTCAAC GT[CT]^[GA]AC
HpaII 1041 CCGG C^CGG
KasI 946 GGCGCC G^GCGCC
MamI 1348 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1146 TGGCCA TGG^CCA
MscI 1146 TGGCCA TGG^CCA
MspA1I 68 CAGCGG C[AC]G^C[GT]G
MspI 1041 CCGG C^CGG
MvnI 1372 CGCG CG^CG
NarI 946 GGCGCC GG^CGCC
NcoI 227 CCATGG C^CATGG
NsiI 542 ATGCAT ATGCA^T
PflFI 1309 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 337 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 102 TCATGA T^CATGA
SfcI 84 CTACAG C^T[GA][CT]AG
SfoI 946 GGCGCC GGC^GCC
SmaI 1040 CCCGGG CCC^GGG
SphI 544 GCATGC GCATG^C
StuI 1125 AGGCCT AGG^CCT
Tth111I 1309 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 337 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 989 CCACCTCGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1040 CCCGGG C^CCGGG
XmaI 1040 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 674 GTAC GT^AC
763 GTAC
AvaI 1040 CCCGGG C^[CT]CG[GA]G
1226 CTCGGG
BanI 946 GGCGCC G^G[CT][GA]CC
1060 GGTGCC
BcnI 1040 CCCGG CC^[GC]GG
1041 CCGGG
BsgI 499 CTGCAC GTGCAG(16/14)
65 GTGCAG
BsoBI 1040 CCCGGG C^[CT]CG[GA]G
1226 CTCGGG
BsrDI 782 GCAATG GCAATG(2/0)
1235 GCAATG
BstAPI 45 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
58 GCAGAAAGTGC
BstNI 697 CCAGG CC^[AT]GG
1383 CCTGG
DpnII 1323 GATC ^GATC
1471 GATC
EcoRII 697 CCAGG ^CC[AT]GG
1383 CCTGG
EcoT14I 227 CCATGG C^C[AT][AT]GG
965 CCTTGG
MboI 1323 GATC ^GATC
1471 GATC
MslI 418 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
434 CATTCCAGTG
MvaI 697 CCAGG CC^[AT]GG
1383 CCTGG
NciI 1040 CCCGG CC^[GC]GG
1041 CCGGG
NdeII 1323 GATC ^GATC
1471 GATC
PleI 1153 GACTC GAGTC(4/5)
1309 GACTC
RsaI 674 GTAC GT^AC
763 GTAC
SacI 755 GAGCTC GAGCT^C
889 GAGCTC
SapI 241 GAAGAGC GCTCTTC(1/4)
1104 GAAGAGC
Sau3AI 1323 GATC ^GATC
1471 GATC
StyI 227 CCATGG C^C[AT][AT]GG
965 CCTTGG
Tsp509I 818 AATT ^AATT
859 AATT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AflIII AluI AspHI AvaII
BanII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspLU11I BsrI BssKI BstF5I Cac8I
CfoI Cfr13I DdeI DsaI EarI
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeIII MboII MnlI MseI
MwoI NlaIII NlaIV NspI Sau96I
ScrFI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I