Restriction map for
pFN21AE1893
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 425 GGTACC G^GTACC
AflIII 758 ACATGT A^C[GA][CT]GT
AhdI 631 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1204 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 425 GGTACC G^GTACC
AspEI 631 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 934 GAGCTC G[AT]GC[AT]^C
AvaI 557 CCCGAG C^[CT]CG[GA]G
BalI 968 TGGCCA TGG^CCA
BanI 425 GGTACC G^G[CT][GA]CC
BsiEI 54 CGACCG CG[GA][CT]^CG
BsiHKAI 934 GAGCTC G[AT]GC[AT]^C
BsoBI 557 CCCGAG C^[CT]CG[GA]G
BspLU11I 758 ACATGT A^CATGT
BspMI 545 ACCTGC ACCTGC(4/8)
BsrBI 813 GAGCGG CCGCTC(-3/-3)
BsrDI 437 CATTGC GCAATG(2/0)
BstYI 160 GGATCT [GA]^GATC[CT]
EaeI 968 TGGCCA [CT]^GGCC[GA]
Eam1105I 631 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 231 GAATTC G^AATTC
HphI 810 GGTGA GGTGA(8/7)
KpnI 425 GGTACC GGTAC^C
MaeII 1079 ACGT A^CGT
MaeIII 98 GTGAC ^GT[ACGT]AC
MflI 160 GGATCT [GA]^GATC[CT]
MluNI 968 TGGCCA TGG^CCA
MscI 968 TGGCCA TGG^CCA
MslI 857 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 454 CAGCTG C[AC]G^C[GT]G
NheI 877 GCTAGC G^CTAGC
NlaIV 425 GGTACC GG[ACGT]^[ACGT]CC
PshAI 395 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 188 CTGCAG CTGCA^G
PvuII 454 CAGCTG CAG^CTG
SacI 934 GAGCTC GAGCT^C
StuI 62 AGGCCT AGG^CCT
Tsp45I 98 GTGAC ^GT[GC]AC
XcmI 1070 CCAAGGCCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 160 GGATCT [GA]^GATC[CT]
XmnI 1204 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 231 GAATTC [GA]^AATT[CT]
1117 AAATTT
AlwI 1005 GATCC GGATC(4/5)
160 GGATC
AlwNI 454 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
997 CAGAATCTG
ApoI 231 GAATTC [GA]^AATT[CT]
1117 AAATTT
AvaII 264 GGTCC G^G[AT]CC
1134 GGACC
BanII 32 GAGCCC G[GA]GC[CT]^C
934 GAGCTC
BmyI 32 GAGCCC G[AGT]GC[ACT]^C
934 GAGCTC
BsmAI 39 GTCTC GTCTC(1/5)
1055 GTCTC
Bsp1286I 32 GAGCCC G[AGT]GC[ACT]^C
934 GAGCTC
FauI 462 GCGGG CCCGC(4/6)
815 GCGGG
HindIII 380 AAGCTT A^AGCTT
820 AAGCTT
MseI 568 TTAA T^TAA
859 TTAA
NspI 632 ACATGC [GA]CATG^[CT]
758 ACATGT
PflMI 378 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
801 CCAGGCTTTGG
SfaNI 146 GCATC GCATC(5/9)
338 GCATC
SfcI 188 CTGCAG C^T[GA][CT]AG
305 CTACAG
Tru9I 568 TTAA T^TAA
859 TTAA
TspRI 125 CAGTG CA[GC]TG[ACGT][ACGT]^
893 CACTG
Van91I 378 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
801 CCAGGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BfaI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII PleI RsaI
Sau3AI Sau96I ScrFI StyI TaqI
TfiI TseI Tsp509I TthHB8I