Restriction map for
pFN21AE3188
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BalI BamHI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 436 GTAGAC GT^[AC][GT]AC
AcsI 617 AAATTC [GA]^AATT[CT]
AlwI 534 GGATC GGATC(4/5)
ApoI 617 AAATTC [GA]^AATT[CT]
Asp700I 613 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 309 CCCGGG C^[CT]CG[GA]G
BcgI 570 CGACACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 185 GCCGCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 737 CTGCAC GTGCAG(16/14)
BsiEI 335 CGGCCG CG[GA][CT]^CG
BsmAI 849 GTCTC GTCTC(1/5)
BsmFI 1057 GGGAC GGGAC(10/14)
BsoBI 309 CCCGGG C^[CT]CG[GA]G
BspMI 212 ACCTGC ACCTGC(4/8)
BsrDI 716 CATTGC GCAATG(2/0)
BstAPI 62 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 534 GGATCT [GA]^GATC[CT]
DpnII 535 GATC ^GATC
DsaI 542 CCATGG C^C[GA][CT]GG
EagI 335 CGGCCG C^GGCCG
EclXI 335 CGGCCG C^GGCCG
Eco52I 335 CGGCCG C^GGCCG
MboI 535 GATC ^GATC
MflI 534 GGATCT [GA]^GATC[CT]
MseI 788 TTAA T^TAA
NcoI 542 CCATGG C^CATGG
NdeII 535 GATC ^GATC
NotI 334 GCGGCCGC GC^GGCCGC
PleI 1033 GACTC GAGTC(4/5)
PvuII 575 CAGCTG CAG^CTG
Sau3AI 535 GATC ^GATC
SexAI 1010 ACCAGGT A^CC[AT]GGT
SmaI 309 CCCGGG CCC^GGG
SmlI 1144 CTTGAG C^T[CT][GA]AG
Tru9I 788 TTAA T^TAA
XcmI 969 CCACTGGGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 534 GGATCT [GA]^GATC[CT]
XmaCI 309 CCCGGG C^CCGGG
XmaI 309 CCCGGG C^CCGGG
XmnI 613 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BbsI 699 GTCTTC GAAGAC(2/6)
924 GTCTTC
BpuAI 699 GTCTTC GAAGAC(2/6)
924 GTCTTC
EarI 384 GAAGAG CTCTTC(1/4)
795 GAAGAG
EcoNI 507 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1143 CCTTGAGGAGG
HgaI 157 GACGC GACGC(5/10)
641 GCGTC
HindIII 1156 AAGCTT A^AGCTT
1189 AAGCTT
Ksp632I 384 GAAGAG CTCTTC(1/4)
795 GAAGAG
PflMI 126 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1011 CCAGGTCCTGG
PpuMI 236 AGGACCT [GA]G^G[AT]CC[CT]
1013 AGGTCCT
SapI 384 GAAGAGC GCTCTTC(1/4)
795 GAAGAGC
Van91I 126 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1011 CCAGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspI AvaII BanI BanII
BbeI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeIII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV PflFI PstI
RsaI Sau96I ScrFI SfaNI SfcI
SfoI StyI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I