Restriction map for
pFN21AE3153
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BsaAI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstUI BstZ17I Bsu36I Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I FauI
FseI FspI HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AfaI 181 GTAC GT^AC
AspHI 262 GAGCTC G[AT]GC[AT]^C
BalI 557 TGGCCA TGG^CCA
BamHI 1052 GGATCC G^GATCC
BanII 262 GAGCTC G[GA]GC[CT]^C
BbsI 906 GTCTTC GAAGAC(2/6)
BclI 269 TGATCA T^GATCA
BcnI 920 CCCGG CC^[GC]GG
BfaI 124 CTAG C^TAG
BglII 875 AGATCT A^GATCT
BlpI 598 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 598 GCTGAGC GC^T[ACGT]AGC
BpuAI 906 GTCTTC GAAGAC(2/6)
BsaBI 789 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 847 GAGACC GGTCTC(1/5)
BsgI 145 GTGCAG GTGCAG(16/14)
BsiHKAI 262 GAGCTC G[AT]GC[AT]^C
BspMI 147 GCAGGT ACCTGC(4/8)
BsrBI 118 GAGCGG CCGCTC(-3/-3)
BsrI 993 CCAGT ACTGG(1/-1)
BstAPI 341 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 234 TTCGAA TT^CGAA
BstEII 957 GGTCACC G^GT[ACGT]ACC
BstPI 957 GGTCACC G^GT[ACGT]ACC
CelII 598 GCTGAGC GC^T[ACGT]AGC
DsaI 686 CCATGG C^C[GA][CT]GG
EaeI 557 TGGCCA [CT]^GGCC[GA]
EcoNI 1032 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 957 GGTCACC G^GT[ACGT]ACC
EcoRI 81 GAATTC G^AATTC
EcoRV 1018 GATATC GAT^ATC
EcoT14I 686 CCATGG C^C[AT][AT]GG
EcoT22I 281 ATGCAT ATGCA^T
FbaI 269 TGATCA T^GATCA
HaeII 602 AGCGCC [GA]GCGC^[CT]
HaeIII 558 GGCC GG^CC
HapII 921 CCGG C^CGG
HpaII 921 CCGG C^CGG
MaeI 124 CTAG C^TAG
MaeII 242 ACGT A^CGT
MamI 789 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
MspI 921 CCGG C^CGG
NciI 920 CCCGG CC^[GC]GG
NcoI 686 CCATGG C^CATGG
NsiI 281 ATGCAT ATGCA^T
NspI 283 GCATGT [GA]CATG^[CT]
NspV 234 TTCGAA TT^CGAA
PstI 839 CTGCAG CTGCA^G
RsaI 181 GTAC GT^AC
SacI 262 GAGCTC GAGCT^C
SfcI 839 CTGCAG C^T[GA][CT]AG
SfuI 234 TTCGAA TT^CGAA
SspI 469 AATATT AAT^ATT
StyI 686 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 120 GCGG CCGC(-3/-1)
924 GCGG
AcyI 12 GACGCC G[GA]^CG[CT]C
913 GACGCC
BsaHI 12 GACGCC G[GA]^CG[CT]C
913 GACGCC
BsmI 543 GAATGC GAATGC(1/-1)
675 GCATTC
BstXI 251 CCACCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
794 CCATCATCGTGG
DdeI 599 CTGAG C^T[ACGT]AG
1098 CTTAG
HgaI 12 GACGC GACGC(5/10)
913 GACGC
Hin1I 12 GACGCC G[GA]^CG[CT]C
913 GACGCC
HincII 478 GTTGAC GT[CT]^[GA]AC
823 GTTGAC
HindII 478 GTTGAC GT[CT]^[GA]AC
823 GTTGAC
HphI 772 GGTGA GGTGA(8/7)
959 TCACC
MaeIII 10 GTGAC ^GT[ACGT]AC
958 GTCAC
MseI 487 TTAA T^TAA
1007 TTAA
TaqI 79 TCGA T^CGA
235 TCGA
TfiI 627 GATTC G^A[AT]TC
738 GATTC
Tru9I 487 TTAA T^TAA
1007 TTAA
Tsp45I 10 GTGAC ^GT[GC]AC
958 GTCAC
TthHB8I 79 TCGA T^CGA
235 TCGA
XcmI 47 CCAAAGGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
941 CCATTGATGAGGTGG
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I CfoI DpnII EarI
EcoRII Fnu4HI FokI HhaI HinP1I
HinfI ItaI Ksp632I MboI MboII
MflI MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI SfaNI TseI Tsp509I TspRI
XhoII