Restriction map for
pFN21AE1728
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BalI BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BpuAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HinfI
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 409 GGCGTC G[GA]^CG[CT]C
AfaI 537 GTAC GT^AC
AhdI 404 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 50 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 404 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 18 CCCGGG C^[CT]CG[GA]G
AvrII 462 CCTAGG C^CTAGG
BanII 652 GAGCCC G[GA]GC[CT]^C
BcgI 239 GCAGGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 463 CTAG C^TAG
BglI 587 GCCCCACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 462 CCTAGG C^CTAGG
BpmI 426 CTCCAG CTGGAG(16/14)
BsaAI 130 TACGTG [CT]AC^GT[GA]
BsaHI 409 GGCGTC G[GA]^CG[CT]C
BseRI 702 GAGGAG GAGGAG(10/8)
BsoBI 18 CCCGGG C^[CT]CG[GA]G
BsrBI 187 CCGCTC CCGCTC(-3/-3)
BsrFI 674 ACCGGC [GA]^CCGG[CT]
BssSI 418 CTCGTG CACGAG(-5/-1)
BstAPI 327 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 674 ACCGGC [GA]^CCGG[CT]
Eam1105I 404 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 483 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 462 CCTAGG C^C[AT][AT]GG
HgaI 410 GCGTC GACGC(5/10)
Hin1I 409 GGCGTC G[GA]^CG[CT]C
KspI 371 CCGCGG CCGC^GG
MaeI 463 CTAG C^TAG
MaeII 131 ACGT A^CGT
PflMI 533 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 691 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 537 GTAC GT^AC
SacII 371 CCGCGG CCGC^GG
SapI 649 GAAGAGC GCTCTTC(1/4)
SfaNI 281 GCATC GCATC(5/9)
SfcI 378 CTACAG C^T[GA][CT]AG
SmaI 18 CCCGGG CCC^GGG
SmlI 484 CTCAAG C^T[CT][GA]AG
SspI 79 AATATT AAT^ATT
StuI 69 AGGCCT AGG^CCT
StyI 462 CCTAGG C^C[AT][AT]GG
Tsp45I 499 GTGAC ^GT[GC]AC
Van91I 533 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 372 CGCG CG^CG
393 CGCG
AspHI 415 GTGCTC G[AT]GC[AT]^C
421 GTGCTC
AvaII 135 GGACC G^G[AT]CC
692 GGTCC
BglII 7 AGATCT A^GATCT
668 AGATCT
BlpI 234 GCTGAGC GC^T[ACGT]AGC
336 GCTGAGC
Bpu1102I 234 GCTGAGC GC^T[ACGT]AGC
336 GCTGAGC
BsiHKAI 415 GTGCTC G[AT]GC[AT]^C
421 GTGCTC
BsmFI 402 GGGAC GGGAC(10/14)
693 GTCCC
BstUI 372 CGCG CG^CG
393 CGCG
BstXI 149 CCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
554 CCAACTACCTGG
CelII 234 GCTGAGC GC^T[ACGT]AGC
336 GCTGAGC
CfoI 309 GCGC GCG^C
394 GCGC
DsaI 371 CCGCGG C^C[GA][CT]GG
590 CCACGG
EarI 3 GAAGAG CTCTTC(1/4)
649 GAAGAG
FauI 227 CCCGC CCCGC(4/6)
370 CCCGC
HhaI 309 GCGC GCG^C
394 GCGC
HinP1I 309 GCGC G^CGC
394 GCGC
Ksp632I 3 GAAGAG CTCTTC(1/4)
649 GAAGAG
MaeIII 31 GTAAC ^GT[ACGT]AC
499 GTGAC
MboII 3 GAAGA GAAGA(8/7)
649 GAAGA
MspA1I 371 CCGCGG C[AC]G^C[GT]G
662 CCGCTG
MvnI 372 CGCG CG^CG
393 CGCG
TspRI 316 CACTG CA[GC]TG[ACGT][ACGT]^
440 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI BamHI BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MboI MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI XhoII