Restriction map for
pFN21AE6613
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwI 585 GGATC GGATC(4/5)
AocI 198 CCTAAGG CC^T[ACGT]AGG
ApaI 494 GGGCCC GGGCC^C
AvaII 125 GGACC G^G[AT]CC
AviII 563 TGCGCA TGC^GCA
BanII 494 GGGCCC G[GA]GC[CT]^C
BbrPI 376 CACGTG CAC^GTG
BcnI 722 CCCGG CC^[GC]GG
BfaI 184 CTAG C^TAG
BpmI 309 CTCCAG CTGGAG(16/14)
BsaAI 376 CACGTG [CT]AC^GT[GA]
BsaJI 351 CCCAGG C^C[ACGT][ACGT]GG
BsgI 499 CTGCAC GTGCAG(16/14)
BspMI 215 ACCTGC ACCTGC(4/8)
BstF5I 615 GGATG GGATG(2/0)
BstNI 352 CCAGG CC^[AT]GG
BstYI 585 GGATCT [GA]^GATC[CT]
Bsu36I 198 CCTAAGG CC^T[ACGT]AGG
DpnII 586 GATC ^GATC
DraIII 205 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 198 CCTAAGG CC^T[ACGT]AGG
EcoRII 352 CCAGG ^CC[AT]GG
FauI 194 CCCGC CCCGC(4/6)
FokI 615 GGATG GGATG(9/13)
FspI 563 TGCGCA TGC^GCA
HphI 429 GGTGA GGTGA(8/7)
MaeI 184 CTAG C^TAG
MaeII 377 ACGT A^CGT
MboI 586 GATC ^GATC
MflI 585 GGATCT [GA]^GATC[CT]
MslI 402 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 485 CAGCTG C[AC]G^C[GT]G
MvaI 352 CCAGG CC^[AT]GG
NciI 722 CCCGG CC^[GC]GG
NdeII 586 GATC ^GATC
NspI 356 GCATGC [GA]CATG^[CT]
PmaCI 376 CACGTG CAC^GTG
PmlI 376 CACGTG CAC^GTG
PstI 230 CTGCAG CTGCA^G
PvuII 485 CAGCTG CAG^CTG
Sau3AI 586 GATC ^GATC
SfcI 230 CTGCAG C^T[GA][CT]AG
SphI 356 GCATGC GCATG^C
TaqI 437 TCGA T^CGA
Tsp45I 430 GTGAC ^GT[GC]AC
Tsp509I 238 AATT ^AATT
TthHB8I 437 TCGA T^CGA
XcmI 352 CCAGGCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 585 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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BalI 380 TGGCCA TGG^CCA
456 TGGCCA
BseRI 69 CTCCTC GAGGAG(10/8)
688 CTCCTC
BsmAI 256 GAGAC GTCTC(1/5)
597 GTCTC
BsmFI 65 GGGAC GGGAC(10/14)
365 GGGAC
BsrDI 471 CATTGC GCAATG(2/0)
7 GCAATG
BsrI 206 ACTGG ACTGG(1/-1)
311 CCAGT
BssKI 352 CCAGG ^CC[ACGT]GG
722 CCCGG
CfoI 142 GCGC GCG^C
564 GCGC
DraII 493 AGGGCCC [GA]G^G[ACGT]CC[CT]
494 GGGCCCT
EaeI 380 TGGCCA [CT]^GGCC[GA]
456 TGGCCA
EarI 622 CTCTTC CTCTTC(1/4)
84 GAAGAG
EcoO109I 493 AGGGCCC [GA]G^G[ACGT]CC[CT]
494 GGGCCCT
HapII 123 CCGG C^CGG
723 CCGG
HhaI 142 GCGC GCG^C
564 GCGC
HinP1I 142 GCGC G^CGC
564 GCGC
HpaII 123 CCGG C^CGG
723 CCGG
Ksp632I 622 CTCTTC CTCTTC(1/4)
84 GAAGAG
MaeIII 430 GTGAC ^GT[ACGT]AC
516 GTTAC
MluNI 380 TGGCCA TGG^CCA
456 TGGCCA
MscI 380 TGGCCA TGG^CCA
456 TGGCCA
MspI 123 CCGG C^CGG
723 CCGG
ScrFI 352 CCAGG CC^[ACGT]GG
722 CCCGG
SfaNI 439 GATGC GCATC(5/9)
416 GCATC
TfiI 300 GATTC G^A[AT]TC
734 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BsiHKAI BsiYI BslI Bsp1286I
Cac8I Cfr13I DdeI Fnu4HI HaeIII
HinfI ItaI MboII MnlI MwoI
NlaIII NlaIV PleI RsaI Sau96I
TseI TspRI