Restriction map for
pFN21AE4335
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaJI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoT14I FauI
FbaI FseI FspI Hin1I KasI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 718 GGTACC G^GTACC
AccII 995 CGCG CG^CG
AciI 623 CCGC CCGC(-3/-1)
AlwI 771 GGATC GGATC(4/5)
Asp718I 718 GGTACC G^GTACC
AvaII 834 GGACC G^G[AT]CC
BanI 718 GGTACC G^G[CT][GA]CC
BanII 987 GGGCTC G[GA]GC[CT]^C
BbsI 409 GAAGAC GAAGAC(2/6)
BbvI 811 GCAGC GCAGC(8/12)
BcnI 1012 CCGGG CC^[GC]GG
BglI 382 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 987 GGGCTC G[AGT]GC[ACT]^C
BpuAI 409 GAAGAC GAAGAC(2/6)
Bsp1286I 987 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 498 TGTACA T^GTACA
BspMI 276 GCAGGT ACCTGC(4/8)
BsrDI 893 GCAATG GCAATG(2/0)
BsrGI 498 TGTACA T^GTACA
BstUI 995 CGCG CG^CG
BstYI 771 GGATCT [GA]^GATC[CT]
CfoI 1009 GCGC GCG^C
DraI 33 TTTAAA TTT^AAA
DrdI 619 GACTCCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 737 CCTCTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 115 GAATTC G^AATTC
EcoRV 747 GATATC GAT^ATC
EcoT22I 449 ATGCAT ATGCA^T
Fnu4HI 811 GCAGC GC^[ACGT]GC
HaeII 1008 AGCGCC [GA]GCGC^[CT]
HapII 1012 CCGG C^CGG
HgaI 996 GCGTC GACGC(5/10)
HhaI 1009 GCGC GCG^C
HinP1I 1009 GCGC G^CGC
HincII 871 GTTAAC GT[CT]^[GA]AC
HindII 871 GTTAAC GT[CT]^[GA]AC
HindIII 84 AAGCTT A^AGCTT
HpaI 871 GTTAAC GTT^AAC
HpaII 1012 CCGG C^CGG
ItaI 811 GCAGC GC^[ACGT]GC
KpnI 718 GGTACC GGTAC^C
MaeIII 568 GTAAC ^GT[ACGT]AC
MflI 771 GGATCT [GA]^GATC[CT]
MspI 1012 CCGG C^CGG
MvnI 995 CGCG CG^CG
NciI 1012 CCGGG CC^[GC]GG
NsiI 449 ATGCAT ATGCA^T
SspBI 498 TGTACA T^GTACA
TseI 811 GCAGC G^C[AT]GC
XbaI 600 TCTAGA T^CTAGA
XcmI 362 CCAACGACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 771 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 115 GAATTC [GA]^AATT[CT]
822 GAATTT
ApoI 115 GAATTC [GA]^AATT[CT]
822 GAATTT
AvaI 98 CTCGGG C^[CT]CG[GA]G
311 CTCGGG
BpmI 352 CTGGAG CTGGAG(16/14)
373 CTGGAG
BsaI 279 GGTCTC GGTCTC(1/5)
492 GGTCTC
BsoBI 98 CTCGGG C^[CT]CG[GA]G
311 CTCGGG
BsrI 405 ACTGG ACTGG(1/-1)
722 CCAGT
Cfr13I 314 GGGCC G^G[ACGT]CC
834 GGACC
DdeI 282 CTCAG C^T[ACGT]AG
1027 CTCAG
DpnII 772 GATC ^GATC
914 GATC
HphI 7 GGTGA GGTGA(8/7)
691 TCACC
MboI 772 GATC ^GATC
914 GATC
MslI 253 CATCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
334 CATAAGTATG
NdeII 772 GATC ^GATC
914 GATC
PleI 309 GACTC GAGTC(4/5)
619 GACTC
Sau3AI 772 GATC ^GATC
914 GATC
Sau96I 314 GGGCC G^G[ACGT]CC
834 GGACC
TaqI 912 TCGA T^CGA
935 TCGA
TfiI 481 GAATC G^A[AT]TC
799 GAATC
TthHB8I 912 TCGA T^CGA
935 TCGA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
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AfaI AluI BfaI BseRI BsiYI
BslI BsmAI BssKI BstF5I BstNI
Cac8I EcoRII FokI HaeIII HinfI
MaeI MboII MnlI MseI MvaI
MwoI NlaIV RsaI ScrFI SfaNI
Tru9I Tsp509I TspRI