Restriction map for
pFN21AE5682
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BbrPI BciVI
BfrI BglII BlnI BsaI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstAPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 30 TCCGGA T^CCGGA
AcsI 581 GAATTT [GA]^AATT[CT]
AcyI 1563 GGCGCC G[GA]^CG[CT]C
AfaI 606 GTAC GT^AC
AlwNI 164 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 581 GAATTT [GA]^AATT[CT]
BamHI 144 GGATCC G^GATCC
BbeI 1563 GGCGCC GGCGC^C
BbsI 3 GAAGAC GAAGAC(2/6)
BbvCI 707 CCTCAGC CCTCAGC(-5/-2)
BclI 1587 TGATCA T^GATCA
BglI 968 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaAI 730 TACGTG [CT]AC^GT[GA]
BsaBI 759 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1563 GGCGCC G[GA]^CG[CT]C
BseAI 30 TCCGGA T^CCGGA
BsmI 775 GCATTC GAATGC(1/-1)
BspEI 30 TCCGGA T^CCGGA
BsrDI 1337 GCAATG GCAATG(2/0)
BssSI 1296 CACGAG CACGAG(-5/-1)
BstBI 653 TTCGAA TT^CGAA
BstXI 227 CCACTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 931 GACTGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1035 CGGCCG C^GGCCG
EarI 874 CTCTTC CTCTTC(1/4)
EclXI 1035 CGGCCG C^GGCCG
Eco52I 1035 CGGCCG C^GGCCG
FbaI 1587 TGATCA T^GATCA
HaeII 1563 GGCGCC [GA]GCGC^[CT]
HgaI 772 GACGC GACGC(5/10)
Hin1I 1563 GGCGCC G[GA]^CG[CT]C
KasI 1563 GGCGCC G^GCGCC
Ksp632I 874 CTCTTC CTCTTC(1/4)
MamI 759 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 30 TCCGGA T^CCGGA
NarI 1563 GGCGCC GG^CGCC
NcoI 383 CCATGG C^CATGG
NspV 653 TTCGAA TT^CGAA
PshAI 34 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 606 GTAC GT^AC
SapI 873 GCTCTTC GCTCTTC(1/4)
SfoI 1563 GGCGCC GGC^GCC
SfuI 653 TTCGAA TT^CGAA
Tsp45I 1231 GTGAC ^GT[GC]AC
Tsp509I 582 AATT ^AATT
XcmI 870 CCAGCTCTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 805 CGCG CG^CG
1514 CGCG
AspHI 787 GAGCTC G[AT]GC[AT]^C
1171 GAGCTC
BalI 734 TGGCCA TGG^CCA
1427 TGGCCA
BanI 1094 GGCACC G^G[CT][GA]CC
1563 GGCGCC
BcgI 835 GCAGCCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1247 GCATCTACTTCG
BlpI 179 GCTCAGC GC^T[ACGT]AGC
1168 GCTGAGC
Bpu1102I 179 GCTCAGC GC^T[ACGT]AGC
1168 GCTGAGC
BseRI 280 GAGGAG GAGGAG(10/8)
1486 GAGGAG
BsgI 1386 CTGCAC GTGCAG(16/14)
1405 CTGCAC
BsiEI 1035 CGGCCG CG[GA][CT]^CG
1268 CGACCG
BsiHKAI 787 GAGCTC G[AT]GC[AT]^C
1171 GAGCTC
BspMI 590 ACCTGC ACCTGC(4/8)
621 GCAGGT
BsrI 781 ACTGG ACTGG(1/-1)
214 CCAGT
BstEII 1201 GGTTACC G^GT[ACGT]ACC
1230 GGTGACC
BstPI 1201 GGTTACC G^GT[ACGT]ACC
1230 GGTGACC
BstUI 805 CGCG CG^CG
1514 CGCG
CelII 179 GCTCAGC GC^T[ACGT]AGC
1168 GCTGAGC
DraII 53 AGGACCC [GA]G^G[ACGT]CC[CT]
209 GGGACCC
DsaI 383 CCATGG C^C[GA][CT]GG
911 CCACGG
EcoO109I 53 AGGACCC [GA]G^G[ACGT]CC[CT]
209 GGGACCC
EcoO65I 1201 GGTTACC G^GT[ACGT]ACC
1230 GGTGACC
EcoT14I 383 CCATGG C^C[AT][AT]GG
1098 CCAAGG
MaeII 731 ACGT A^CGT
1520 ACGT
MaeIII 1202 GTTAC ^GT[ACGT]AC
1231 GTGAC
MluNI 734 TGGCCA TGG^CCA
1427 TGGCCA
MscI 734 TGGCCA TGG^CCA
1427 TGGCCA
MslI 817 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1126 CATCCCGATG
MvnI 805 CGCG CG^CG
1514 CGCG
NlaIII 384 CATG CATG^
610 CATG
PpuMI 53 AGGACCC [GA]G^G[AT]CC[CT]
209 GGGACCC
PstI 314 CTGCAG CTGCA^G
410 CTGCAG
PvuII 182 CAGCTG CAG^CTG
454 CAGCTG
SacI 787 GAGCTC GAGCT^C
1171 GAGCTC
StyI 383 CCATGG C^C[AT][AT]GG
1098 CCAAGG
TfiI 275 GAATC G^A[AT]TC
759 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I XhoII