Restriction map for
pFN21AE2045
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BciVI BfrI BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspMI BsrBI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoRV EcoT22I
FseI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SwaI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Alw44I 1605 GTGCAC G^TGCAC
ApaLI 1605 GTGCAC G^TGCAC
Asp700I 772 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 194 TGCGCA TGC^GCA
BbrPI 1602 CACGTG CAC^GTG
BbvCI 1102 GCTGAGG CCTCAGC(-5/-2)
BcgI 1785 GCATCTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1300 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1300 GCTCAGC GC^T[ACGT]AGC
BsaAI 1602 CACGTG [CT]AC^GT[GA]
BsmBI 723 GAGACG CGTCTC(1/5)
Bsp1407I 1275 TGTACA T^GTACA
BspHI 150 TCATGA T^CATGA
BspLU11I 365 ACATGT A^CATGT
BsrDI 1410 CATTGC GCAATG(2/0)
BsrFI 120 GCCGGC [GA]^CCGG[CT]
BsrGI 1275 TGTACA T^GTACA
BssSI 1063 CACGAG CACGAG(-5/-1)
BstEII 440 GGTTACC G^GT[ACGT]ACC
BstPI 440 GGTTACC G^GT[ACGT]ACC
CelII 1300 GCTCAGC GC^T[ACGT]AGC
Cfr10I 120 GCCGGC [GA]^CCGG[CT]
EagI 118 CGGCCG C^GGCCG
EclXI 118 CGGCCG C^GGCCG
Eco52I 118 CGGCCG C^GGCCG
EcoNI 321 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 440 GGTTACC G^GT[ACGT]ACC
EcoRI 848 GAATTC G^AATTC
FspI 194 TGCGCA TGC^GCA
HincII 1568 GTCAAC GT[CT]^[GA]AC
HindII 1568 GTCAAC GT[CT]^[GA]AC
MluI 221 ACGCGT A^CGCGT
NaeI 120 GCCGGC GCC^GGC
NgoMIV 120 GCCGGC G^CCGGC
PmaCI 1602 CACGTG CAC^GTG
PmlI 1602 CACGTG CAC^GTG
RcaI 150 TCATGA T^CATGA
SacI 582 GAGCTC GAGCT^C
SexAI 1094 ACCTGGT A^CC[AT]GGT
SfiI 24 GGCCACCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1202 CCCGGG CCC^GGG
SphI 1617 GCATGC GCATG^C
SspBI 1275 TGTACA T^GTACA
SspI 1838 AATATT AAT^ATT
XcmI 690 CCAGTTCCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1202 CCCGGG C^CCGGG
XmaI 1202 CCCGGG C^CCGGG
XmnI 772 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 54 GTAC GT^AC
1276 GTAC
ApaI 252 GGGCCC GGGCC^C
1205 GGGCCC
BclI 147 TGATCA T^GATCA
609 TGATCA
BfaI 598 CTAG C^TAG
751 CTAG
BglI 25 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1815 GCCTCTGGGGC
BglII 410 AGATCT A^GATCT
614 AGATCT
BsiEI 118 CGGCCG CG[GA][CT]^CG
234 CGACCG
DraIII 1093 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1587 CACCATGTG
DsaI 938 CCATGG C^C[GA][CT]GG
1187 CCATGG
FbaI 147 TGATCA T^GATCA
609 TGATCA
HphI 1586 TCACC GGTGA(8/7)
1982 TCACC
MaeI 598 CTAG C^TAG
751 CTAG
NcoI 938 CCATGG C^CATGG
1187 CCATGG
NspI 365 ACATGT [GA]CATG^[CT]
1617 GCATGC
PflMI 204 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1775 CCATCTCATGG
PpuMI 1472 GGGACCC [GA]G^G[AT]CC[CT]
1658 GGGTCCC
RsaI 54 GTAC GT^AC
1276 GTAC
SapI 772 GAAGAGC GCTCTTC(1/4)
39 GCTCTTC
StuI 1487 AGGCCT AGG^CCT
1757 AGGCCT
TaqI 381 TCGA T^CGA
730 TCGA
TfiI 494 GATTC G^A[AT]TC
951 GATTC
TthHB8I 381 TCGA T^CGA
730 TCGA
Van91I 204 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1775 CCATCTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I Tsp509I TspRI
XhoII