Restriction map for
pFN21AE9541
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII ClaI CpoI DraI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1327 GTAGAC GT^[AC][GT]AC
AcyI 926 GGCGCC G[GA]^CG[CT]C
AflIII 1301 ACGTGT A^C[GA][CT]GT
AocI 805 CCTGAGG CC^T[ACGT]AGG
BamHI 706 GGATCC G^GATCC
BbeI 926 GGCGCC GGCGC^C
BfaI 487 CTAG C^TAG
BsaHI 926 GGCGCC G[GA]^CG[CT]C
BseRI 721 GAGGAG GAGGAG(10/8)
BsiEI 1213 CGGCCG CG[GA][CT]^CG
BstAPI 595 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 192 GGTGACC G^GT[ACGT]ACC
BstPI 192 GGTGACC G^GT[ACGT]ACC
Bsu36I 805 CCTGAGG CC^T[ACGT]AGG
DrdI 802 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1213 CGGCCG C^GGCCG
EclXI 1213 CGGCCG C^GGCCG
Eco52I 1213 CGGCCG C^GGCCG
Eco81I 805 CCTGAGG CC^T[ACGT]AGG
EcoO65I 192 GGTGACC G^GT[ACGT]ACC
Hin1I 926 GGCGCC G[GA]^CG[CT]C
KasI 926 GGCGCC G^GCGCC
MaeI 487 CTAG C^TAG
NaeI 1215 GCCGGC GCC^GGC
NarI 926 GGCGCC GG^CGCC
NdeI 1169 CATATG CA^TATG
NgoMIV 1215 GCCGGC G^CCGGC
NspI 1046 GCATGC [GA]CATG^[CT]
PaeR7I 508 CTCGAG C^TCGAG
PstI 1115 CTGCAG CTGCA^G
SfaNI 1292 GCATC GCATC(5/9)
SfcI 1115 CTGCAG C^T[GA][CT]AG
SfoI 926 GGCGCC GGC^GCC
SmaI 216 CCCGGG CCC^GGG
SmlI 508 CTCGAG C^T[CT][GA]AG
SphI 1046 GCATGC GCATG^C
XcmI 1112 CCACTGCAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 508 CTCGAG C^TCGAG
XmaCI 216 CCCGGG C^CCGGG
XmaI 216 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 250 GTAC GT^AC
377 GTAC
AvaI 216 CCCGGG C^[CT]CG[GA]G
508 CTCGAG
BalI 989 TGGCCA TGG^CCA
1238 TGGCCA
BbrPI 301 CACGTG CAC^GTG
1300 CACGTG
BsaAI 301 CACGTG [CT]AC^GT[GA]
1300 CACGTG
BsaI 454 GGTCTC GGTCTC(1/5)
637 GGTCTC
BsgI 898 GTGCAG GTGCAG(16/14)
1005 GTGCAG
BsmAI 455 GTCTC GTCTC(1/5)
638 GTCTC
BsmFI 235 GTCCC GGGAC(10/14)
1011 GTCCC
BsoBI 216 CCCGGG C^[CT]CG[GA]G
508 CTCGAG
BsrFI 1215 GCCGGC [GA]^CCGG[CT]
1267 GCCGGT
BstYI 86 GGATCT [GA]^GATC[CT]
706 GGATCC
Cfr10I 1215 GCCGGC [GA]^CCGG[CT]
1267 GCCGGT
DpnII 87 GATC ^GATC
707 GATC
DraIII 298 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
328 CACTCTGTG
DsaI 831 CCATGG C^C[GA][CT]GG
992 CCATGG
EarI 727 GAAGAG CTCTTC(1/4)
742 GAAGAG
EcoNI 120 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
444 CCTGGGTCAGG
HphI 192 GGTGA GGTGA(8/7)
863 TCACC
Ksp632I 727 GAAGAG CTCTTC(1/4)
742 GAAGAG
MaeII 302 ACGT A^CGT
1301 ACGT
MboI 87 GATC ^GATC
707 GATC
MflI 86 GGATCT [GA]^GATC[CT]
706 GGATCC
MluNI 989 TGGCCA TGG^CCA
1238 TGGCCA
MscI 989 TGGCCA TGG^CCA
1238 TGGCCA
NcoI 831 CCATGG C^CATGG
992 CCATGG
NdeII 87 GATC ^GATC
707 GATC
PflMI 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
327 CCACTCTGTGG
PmaCI 301 CACGTG CAC^GTG
1300 CACGTG
PmlI 301 CACGTG CAC^GTG
1300 CACGTG
PpuMI 233 AGGTCCC [GA]G^G[AT]CC[CT]
1009 AGGTCCC
RsaI 250 GTAC GT^AC
377 GTAC
Sau3AI 87 GATC ^GATC
707 GATC
TaqI 509 TCGA T^CGA
554 TCGA
TthHB8I 509 TCGA T^CGA
554 TCGA
Van91I 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
327 CCACTCTGTGG
XhoII 86 GGATCT [GA]^GATC[CT]
706 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BcnI BglI
BmyI BsaJI BsiYI BslI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PvuII Sau96I ScrFI StyI
TseI Tsp45I TspRI