Restriction map for
pFN21AE2175
##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TspRI Tth111I Van91I XbaI XcmI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 329 CGCG CG^CG
AcsI 50 AAATTC [GA]^AATT[CT]
AluI 59 AGCT AG^CT
AlwNI 334 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 296 AGCGCT AGC^GCT
ApoI 50 AAATTC [GA]^AATT[CT]
Asp700I 129 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 12 CCCGGG C^[CT]CG[GA]G
AvaII 117 GGACC G^G[AT]CC
BbsI 142 GAAGAC GAAGAC(2/6)
BpuAI 142 GAAGAC GAAGAC(2/6)
BseRI 304 GAGGAG GAGGAG(10/8)
BsgI 352 GTGCAG GTGCAG(16/14)
BsiYI 278 CCCGCAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 278 CCCGCAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 221 GAGAC GTCTC(1/5)
BsmFI 248 GGGAC GGGAC(10/14)
BsoBI 12 CCCGGG C^[CT]CG[GA]G
BspMI 234 GCAGGT ACCTGC(4/8)
BsrFI 21 GCCGGT [GA]^CCGG[CT]
BstEII 24 GGTCACC G^GT[ACGT]ACC
BstF5I 9 CATCC GGATG(2/0)
BstPI 24 GGTCACC G^GT[ACGT]ACC
BstUI 329 CGCG CG^CG
CfoI 297 GCGC GCG^C
Cfr10I 21 GCCGGT [GA]^CCGG[CT]
DpnII 228 GATC ^GATC
DrdI 327 GACGCGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 296 AGCGCT AGC^GCT
EcoO65I 24 GGTCACC G^GT[ACGT]ACC
EcoT14I 257 CCATGG C^C[AT][AT]GG
FauI 278 CCCGC CCCGC(4/6)
FokI 9 CATCC GGATG(9/13)
HaeII 296 AGCGCT [GA]GCGC^[CT]
HhaI 297 GCGC GCG^C
HinP1I 297 GCGC G^CGC
MboI 228 GATC ^GATC
MboII 142 GAAGA GAAGA(8/7)
MseI 79 TTAA T^TAA
MvnI 329 CGCG CG^CG
NcoI 257 CCATGG C^CATGG
NdeII 228 GATC ^GATC
NlaIV 117 GGACCC GG[ACGT]^[ACGT]CC
PpuMI 116 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 255 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 228 GATC ^GATC
SmaI 12 CCCGGG CCC^GGG
StyI 257 CCATGG C^C[AT][AT]GG
TaqI 110 TCGA T^CGA
Tru9I 79 TTAA T^TAA
Tsp509I 51 AATT ^AATT
TthHB8I 110 TCGA T^CGA
XmaCI 12 CCCGGG C^CCGGG
XmaI 12 CCCGGG C^CCGGG
XmnI 129 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BcnI 12 CCCGG CC^[GC]GG
13 CCGGG
BstAPI 333 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
357 GCAGTCCATGC
DraII 116 AGGACCC [GA]G^G[ACGT]CC[CT]
189 AGGCCCT
DsaI 32 CCGTGG C^C[GA][CT]GG
257 CCATGG
EcoO109I 116 AGGACCC [GA]G^G[ACGT]CC[CT]
189 AGGCCCT
HapII 13 CCGG C^CGG
22 CCGG
HinfI 107 GAATC G^A[ACGT]TC
129 GAATC
HpaII 13 CCGG C^CGG
22 CCGG
MspI 13 CCGG C^CGG
22 CCGG
NciI 12 CCCGG CC^[GC]GG
13 CCGGG
PstI 211 CTGCAG CTGCA^G
340 CTGCAG
SfcI 211 CTGCAG C^T[GA][CT]AG
340 CTGCAG
TfiI 107 GAATC G^A[AT]TC
129 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI BbvI BsaJI BssKI BstNI
Cac8I Cfr13I EcoRII Fnu4HI HaeIII
HgaI HphI ItaI MaeIII MnlI
MslI MvaI MwoI NlaIII Sau96I
ScrFI TseI Tsp45I