Restriction map for
pFN21AE5631
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AhdI 211 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1018 GTGCAC G^TGCAC
ApaLI 1018 GTGCAC G^TGCAC
AspEI 211 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1391 TGGCCA TGG^CCA
BamHI 1242 GGATCC G^GATCC
BbrPI 355 CACGTG CAC^GTG
BlpI 1257 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1257 GCTGAGC GC^T[ACGT]AGC
BseRI 6 CTCCTC GAGGAG(10/8)
BsgI 1369 GTGCAG GTGCAG(16/14)
BsiEI 890 CGGCCG CG[GA][CT]^CG
BspMI 98 ACCTGC ACCTGC(4/8)
BsrBI 666 GAGCGG CCGCTC(-3/-3)
BstAPI 1089 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1242 GGATCC [GA]^GATC[CT]
CelII 1257 GCTGAGC GC^T[ACGT]AGC
DraIII 1012 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 890 CGGCCG C^GGCCG
Eam1105I 211 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 890 CGGCCG C^GGCCG
Eco52I 890 CGGCCG C^GGCCG
HgaI 895 GACGC GACGC(5/10)
HinfI 643 GACTC G^A[ACGT]TC
MflI 1242 GGATCC [GA]^GATC[CT]
MluNI 1391 TGGCCA TGG^CCA
MscI 1391 TGGCCA TGG^CCA
PaeR7I 1138 CTCGAG C^TCGAG
PleI 643 GACTC GAGTC(4/5)
PmaCI 355 CACGTG CAC^GTG
PmlI 355 CACGTG CAC^GTG
PstI 157 CTGCAG CTGCA^G
SacI 3 GAGCTC GAGCT^C
SfiI 226 GGCCTCGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1138 CTCGAG C^T[CT][GA]AG
StuI 225 AGGCCT AGG^CCT
XhoI 1138 CTCGAG C^TCGAG
XhoII 1242 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 876 CGCG CG^CG
897 CGCG
AcyI 28 GGCGCC G[GA]^CG[CT]C
1378 GGCGCC
ApaI 635 GGGCCC GGGCC^C
713 GGGCCC
AspI 856 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
895 GACGCGGTC
BbeI 28 GGCGCC GGCGC^C
1378 GGCGCC
BbsI 1111 GAAGAC GAAGAC(2/6)
491 GTCTTC
BpuAI 1111 GAAGAC GAAGAC(2/6)
491 GTCTTC
BsaAI 355 CACGTG [CT]AC^GT[GA]
544 TACGTG
BsaHI 28 GGCGCC G[GA]^CG[CT]C
1378 GGCGCC
BsaWI 332 ACCGGA [AT]^CCGG[AT]
580 TCCGGT
BsrI 467 ACTGG ACTGG(1/-1)
432 CCAGT
BstEII 840 GGTGACC G^GT[ACGT]ACC
1069 GGTTACC
BstPI 840 GGTGACC G^GT[ACGT]ACC
1069 GGTTACC
BstUI 876 CGCG CG^CG
897 CGCG
BstXI 824 CCACAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1277 CCATTGACGTGG
DrdI 451 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
541 GACTACGTGGTC
EarI 655 CTCTTC CTCTTC(1/4)
485 GAAGAG
EcoO65I 840 GGTGACC G^GT[ACGT]ACC
1069 GGTTACC
Hin1I 28 GGCGCC G[GA]^CG[CT]C
1378 GGCGCC
KasI 28 GGCGCC G^GCGCC
1378 GGCGCC
Ksp632I 655 CTCTTC CTCTTC(1/4)
485 GAAGAG
MvnI 876 CGCG CG^CG
897 CGCG
NarI 28 GGCGCC GG^CGCC
1378 GGCGCC
NcoI 410 CCATGG C^CATGG
1331 CCATGG
PflFI 856 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
895 GACGCGGTC
PflMI 201 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
432 CCAGTGGATGG
SfoI 28 GGCGCC GGC^GCC
1378 GGCGCC
Tsp45I 592 GTCAC ^GT[GC]AC
841 GTGAC
Tth111I 856 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
895 GACGCGGTC
Van91I 201 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
432 CCAGTGGATGG
XcmI 347 CCATCCCCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1331 CCATGGGGCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TseI TspRI TthHB8I XmaCI XmaI