Restriction map for
pFN21AE4157
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AccI AccIII AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbsI BclI
BfaI BfrI BglI BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I FbaI FseI FspI HincII
HindII HpaI KasI KspI MaeI
MamI MfeI MluI MroI MslI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 487 GACGTC GACGT^C
Acc65I 1612 GGTACC G^GTACC
AclI 562 AACGTT AA^CGTT
AlwNI 700 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 54 AGCGCT AGC^GCT
Asp718I 1612 GGTACC G^GTACC
AvaI 803 CCCGGG C^[CT]CG[GA]G
BalI 788 TGGCCA TGG^CCA
BcgI 877 CGATGCATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 407 GTATCC GTATCC(6/5)
BglII 1599 AGATCT A^GATCT
BmyI 885 GTGCCC G[AGT]GC[ACT]^C
BsaAI 1627 TACGTG [CT]AC^GT[GA]
BsmBI 485 GAGACG CGTCTC(1/5)
BsoBI 803 CCCGGG C^[CT]CG[GA]G
Bsp1286I 885 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 1127 TGTACA T^GTACA
BspMI 329 ACCTGC ACCTGC(4/8)
BsrGI 1127 TGTACA T^GTACA
DraII 1330 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 841 GAAGAG CTCTTC(1/4)
Eco47III 54 AGCGCT AGC^GCT
EcoO109I 1330 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 505 GAATTC G^AATTC
EcoRV 231 GATATC GAT^ATC
FauI 1537 CCCGC CCCGC(4/6)
HaeII 54 AGCGCT [GA]GCGC^[CT]
HphI 1150 GGTGA GGTGA(8/7)
KpnI 1612 GGTACC GGTAC^C
Ksp632I 841 GAAGAG CTCTTC(1/4)
MluNI 788 TGGCCA TGG^CCA
MscI 788 TGGCCA TGG^CCA
MspA1I 323 CAGCTG C[AC]G^C[GT]G
NdeI 1350 CATATG CA^TATG
PpuMI 1330 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1221 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 562 AACGTT AA^CGTT
PstI 1747 CTGCAG CTGCA^G
PvuII 323 CAGCTG CAG^CTG
SexAI 98 ACCTGGT A^CC[AT]GGT
SmaI 803 CCCGGG CCC^GGG
SmlI 550 CTTGAG C^T[CT][GA]AG
SphI 938 GCATGC GCATG^C
SspBI 1127 TGTACA T^GTACA
XcmI 458 CCACAGAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 803 CCCGGG C^CCGGG
XmaI 803 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 505 GAATTC [GA]^AATT[CT]
1524 GAATTT
AcyI 487 GACGTC G[GA]^CG[CT]C
1441 GACGCC
ApoI 505 GAATTC [GA]^AATT[CT]
1524 GAATTT
BanI 1612 GGTACC G^G[CT][GA]CC
1661 GGCACC
BbvCI 28 CCTCAGC CCTCAGC(-5/-2)
4 GCTGAGG
BlpI 57 GCTCAGC GC^T[ACGT]AGC
756 GCTCAGC
BpmI 681 CTCCAG CTGGAG(16/14)
469 CTGGAG
Bpu1102I 57 GCTCAGC GC^T[ACGT]AGC
756 GCTCAGC
BsaHI 487 GACGTC G[GA]^CG[CT]C
1441 GACGCC
BsgI 127 GTGCAG GTGCAG(16/14)
1709 GTGCAG
BsrBI 754 CCGCTC CCGCTC(-3/-3)
1379 GAGCGG
CelII 57 GCTCAGC GC^T[ACGT]AGC
756 GCTCAGC
DraI 1495 TTTAAA TTT^AAA
1639 TTTAAA
EaeI 149 CGGCCA [CT]^GGCC[GA]
788 TGGCCA
EcoT22I 879 ATGCAT ATGCA^T
1530 ATGCAT
HapII 124 CCGG C^CGG
804 CCGG
Hin1I 487 GACGTC G[GA]^CG[CT]C
1441 GACGCC
HpaII 124 CCGG C^CGG
804 CCGG
MspI 124 CCGG C^CGG
804 CCGG
NsiI 879 ATGCAT ATGCA^T
1530 ATGCAT
NspI 881 GCATGT [GA]CATG^[CT]
938 GCATGC
Tsp45I 413 GTGAC ^GT[GC]AC
826 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BcnI BsaJI BseRI
BsiYI BslI BsmAI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HindIII
HinfI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII