Restriction map for
pFN21AE6748
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# NO CUT ENZYMES ( 127 enzymes) #
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Acc65I AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbvCI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsiEI BsiHKAI
BsiWI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HpaI KasI KpnI KspI MaeI
MfeI MluI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 964 GACGTC GACGT^C
AccI 1177 GTCTAC GT^[AC][GT]AC
AfaI 613 GTAC GT^AC
AlwNI 448 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1065 CCTCAGG CC^T[ACGT]AGG
BanI 1393 GGTGCC G^G[CT][GA]CC
BbrPI 1090 CACGTG CAC^GTG
BcgI 153 CGACGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 374 TGATCA T^GATCA
BsaBI 375 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 418 GAGACC GGTCTC(1/5)
BsgI 883 GTGCAG GTGCAG(16/14)
BsmBI 188 GAGACG CGTCTC(1/5)
BsmI 1101 GAATGC GAATGC(1/-1)
BspHI 383 TCATGA T^CATGA
BsrBI 681 CCGCTC CCGCTC(-3/-3)
BsrDI 969 CATTGC GCAATG(2/0)
BsrI 1565 ACTGG ACTGG(1/-1)
BstAPI 876 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 708 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1065 CCTCAGG CC^T[ACGT]AGG
CfoI 1387 GCGC GCG^C
DrdI 190 GACGGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1065 CCTCAGG CC^T[ACGT]AGG
FbaI 374 TGATCA T^GATCA
HgaI 904 GACGC GACGC(5/10)
HhaI 1387 GCGC GCG^C
HinP1I 1387 GCGC G^CGC
HincII 928 GTCAAC GT[CT]^[GA]AC
HindII 928 GTCAAC GT[CT]^[GA]AC
MamI 375 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PmaCI 1090 CACGTG CAC^GTG
PmlI 1090 CACGTG CAC^GTG
PstI 82 CTGCAG CTGCA^G
PvuII 34 CAGCTG CAG^CTG
RcaI 383 TCATGA T^CATGA
RsaI 613 GTAC GT^AC
TfiI 69 GATTC G^A[AT]TC
TspRI 1279 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 408 CGCG CG^CG
1386 CGCG
AccIII 294 TCCGGA T^CCGGA
794 TCCGGA
AcsI 1119 AAATTT [GA]^AATT[CT]
1192 GAATTT
AcyI 904 GACGCC G[GA]^CG[CT]C
964 GACGTC
ApoI 1119 AAATTT [GA]^AATT[CT]
1192 GAATTT
BanII 639 GAGCCC G[GA]GC[CT]^C
1628 GGGCTC
BsaAI 487 TACGTG [CT]AC^GT[GA]
1090 CACGTG
BsaHI 904 GACGCC G[GA]^CG[CT]C
964 GACGTC
BsaWI 294 TCCGGA [AT]^CCGG[AT]
794 TCCGGA
BseAI 294 TCCGGA T^CCGGA
794 TCCGGA
BseRI 1631 CTCCTC GAGGAG(10/8)
835 GAGGAG
BspEI 294 TCCGGA T^CCGGA
794 TCCGGA
BstUI 408 CGCG CG^CG
1386 CGCG
BstYI 599 AGATCC [GA]^GATC[CT]
1533 GGATCT
EcoNI 462 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1017 CCTGAACCAGG
Hin1I 904 GACGCC G[GA]^CG[CT]C
964 GACGTC
HindIII 323 AAGCTT A^AGCTT
1485 AAGCTT
MflI 599 AGATCC [GA]^GATC[CT]
1533 GGATCT
MroI 294 TCCGGA T^CCGGA
794 TCCGGA
MslI 520 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
654 CACAGAGGTG
MvnI 408 CGCG CG^CG
1386 CGCG
NcoI 908 CCATGG C^CATGG
1539 CCATGG
SapI 636 GAAGAGC GCTCTTC(1/4)
999 GCTCTTC
SfcI 82 CTGCAG C^T[GA][CT]AG
1268 CTACAG
SmlI 745 CTTGAG C^T[CT][GA]AG
772 CTCAAG
SspI 9 AATATT AAT^ATT
244 AATATT
XhoII 599 AGATCC [GA]^GATC[CT]
1533 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TthHB8I