Restriction map for
pFN21AE1153
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MroI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tsp45I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Alw44I 890 GTGCAC G^TGCAC
ApaLI 890 GTGCAC G^TGCAC
Asp700I 1282 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 879 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 716 TGGCCA TGG^CCA
BanI 86 GGCGCC G^G[CT][GA]CC
BbeI 86 GGCGCC GGCGC^C
BbsI 1312 GAAGAC GAAGAC(2/6)
BglI 718 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 370 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 370 GCTGAGC GC^T[ACGT]AGC
BpuAI 1312 GAAGAC GAAGAC(2/6)
BsgI 1384 GTGCAG GTGCAG(16/14)
BsrFI 212 GCCGGT [GA]^CCGG[CT]
CelII 370 GCTGAGC GC^T[ACGT]AGC
Cfr10I 212 GCCGGT [GA]^CCGG[CT]
DraIII 283 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 716 TGGCCA [CT]^GGCC[GA]
HincII 1438 GTTAAC GT[CT]^[GA]AC
HindII 1438 GTTAAC GT[CT]^[GA]AC
HpaI 1438 GTTAAC GTT^AAC
KasI 86 GGCGCC G^GCGCC
MluNI 716 TGGCCA TGG^CCA
MscI 716 TGGCCA TGG^CCA
NarI 86 GGCGCC GG^CGCC
PflFI 879 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 551 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1048 CTGCAG CTGCA^G
SacI 373 GAGCTC GAGCT^C
ScaI 1108 AGTACT AGT^ACT
SfoI 86 GGCGCC GGC^GCC
SspI 589 AATATT AAT^ATT
StuI 1125 AGGCCT AGG^CCT
Tth111I 879 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1282 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 74 GGGCCC GGGCC^C
108 GGGCCC
BsaI 643 GAGACC GGTCTC(1/5)
987 GAGACC
BssSI 91 CACGAG CACGAG(-5/-1)
184 CACGAG
BstYI 233 AGATCC [GA]^GATC[CT]
933 GGATCT
EarI 145 GAAGAG CTCTTC(1/4)
1273 GAAGAG
EcoT14I 269 CCTTGG C^C[AT][AT]GG
548 CCAAGG
HgaI 691 GACGC GACGC(5/10)
55 GCGTC
HphI 1030 GGTGA GGTGA(8/7)
967 TCACC
Ksp632I 145 GAAGAG CTCTTC(1/4)
1273 GAAGAG
MflI 233 AGATCC [GA]^GATC[CT]
933 GGATCT
MslI 682 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
846 CATTGGAATG
NdeI 541 CATATG CA^TATG
638 CATATG
PvuII 41 CAGCTG CAG^CTG
1448 CAGCTG
SfaNI 660 GATGC GCATC(5/9)
964 GCATC
SfcI 1048 CTGCAG C^T[GA][CT]AG
1352 CTGTAG
StyI 269 CCTTGG C^C[AT][AT]GG
548 CCAAGG
TaqI 449 TCGA T^CGA
597 TCGA
TthHB8I 449 TCGA T^CGA
597 TCGA
XcmI 455 CCAGTAGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1185 CCAACCCCTCCCTGG
XhoII 233 AGATCC [GA]^GATC[CT]
933 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SmlI
TfiI Tru9I TseI Tsp509I TspRI