Restriction map for
pFN21AE4043
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
EarI Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MslI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 25 GGCGTC G[GA]^CG[CT]C
AluI 607 AGCT AG^CT
Aor51HI 101 AGCGCT AGC^GCT
BalI 647 TGGCCA TGG^CCA
BclI 399 TGATCA T^GATCA
BfaI 82 CTAG C^TAG
BglII 566 AGATCT A^GATCT
BsaHI 25 GGCGTC G[GA]^CG[CT]C
BsaI 240 GAGACC GGTCTC(1/5)
BsgI 502 CTGCAC GTGCAG(16/14)
BsiEI 507 CGGCCG CG[GA][CT]^CG
BsmBI 544 GAGACG CGTCTC(1/5)
BsmI 666 GAATGC GAATGC(1/-1)
BspHI 29 TCATGA T^CATGA
BspMI 441 GCAGGT ACCTGC(4/8)
BsrDI 137 GCAATG GCAATG(2/0)
BssSI 709 CACGAG CACGAG(-5/-1)
BstAPI 441 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 72 CCATGG C^C[GA][CT]GG
EagI 507 CGGCCG C^GGCCG
EclXI 507 CGGCCG C^GGCCG
Eco47III 101 AGCGCT AGC^GCT
Eco52I 507 CGGCCG C^GGCCG
EcoNI 591 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 72 CCATGG C^C[AT][AT]GG
FbaI 399 TGATCA T^GATCA
Hin1I 25 GGCGTC G[GA]^CG[CT]C
MaeI 82 CTAG C^TAG
MluNI 647 TGGCCA TGG^CCA
MscI 647 TGGCCA TGG^CCA
NcoI 72 CCATGG C^CATGG
NlaIV 704 GGCCCC GG[ACGT]^[ACGT]CC
PshAI 33 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 448 CTGCAG CTGCA^G
RcaI 29 TCATGA T^CATGA
ScaI 653 AGTACT AGT^ACT
SexAI 392 ACCTGGT A^CC[AT]GGT
SfaNI 630 GATGC GCATC(5/9)
SfcI 448 CTGCAG C^T[GA][CT]AG
SgrAI 43 CACCGGCG C[GA]^CCGG[CT]G
StyI 72 CCATGG C^C[AT][AT]GG
TfiI 261 GAATC G^A[AT]TC
Tsp509I 279 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 558 CGCG CG^CG
677 CGCG
AfaI 549 GTAC GT^AC
654 GTAC
AlwI 714 GATCC GGATC(4/5)
623 GGATC
AlwNI 58 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
442 CAGGTGCTG
AvaII 126 GGTCC G^G[AT]CC
785 GGTCC
BbsI 372 GTCTTC GAAGAC(2/6)
724 GTCTTC
BcnI 510 CCGGG CC^[GC]GG
540 CCGGG
BpmI 55 CTCCAG CTGGAG(16/14)
520 CTGGAG
BpuAI 372 GTCTTC GAAGAC(2/6)
724 GTCTTC
BsaJI 72 CCATGG C^C[ACGT][ACGT]GG
597 CCAGGG
BseRI 589 CTCCTC GAGGAG(10/8)
218 GAGGAG
BsmAI 240 GAGAC GTCTC(1/5)
544 GAGAC
BsmFI 92 GGGAC GGGAC(10/14)
777 GTCCC
BsrFI 44 ACCGGC [GA]^CCGG[CT]
361 ACCGGC
BstUI 558 CGCG CG^CG
677 CGCG
BstYI 566 AGATCT [GA]^GATC[CT]
713 AGATCC
Cac8I 77 GCGTGC GC[ACGT]^[ACGT]GC
104 GCTTGC
Cfr10I 44 ACCGGC [GA]^CCGG[CT]
361 ACCGGC
DdeI 354 CTCAG C^T[ACGT]AG
734 CTGAG
EaeI 507 CGGCCG [CT]^GGCC[GA]
647 TGGCCA
HaeII 47 GGCGCT [GA]GCGC^[CT]
101 AGCGCT
MaeII 95 ACGT A^CGT
547 ACGT
MaeIII 223 GTCAC ^GT[ACGT]AC
581 GTCAC
MflI 566 AGATCT [GA]^GATC[CT]
713 AGATCC
MseI 179 TTAA T^TAA
407 TTAA
MvnI 558 CGCG CG^CG
677 CGCG
NciI 510 CCGGG CC^[GC]GG
540 CCGGG
RsaI 549 GTAC GT^AC
654 GTAC
Tru9I 179 TTAA T^TAA
407 TTAA
Tsp45I 223 GTCAC ^GT[GC]AC
581 GTCAC
XcmI 245 CCATTTATGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
515 CCATCCTGGAGATGG
XhoII 566 AGATCT [GA]^GATC[CT]
713 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI BbvI BsiYI BslI BsrI
BssKI BstF5I BstNI CfoI Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII PleI Sau3AI Sau96I ScrFI
TaqI TseI TspRI TthHB8I