################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AhdI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 120 GGTACC G^GTACC AfaI 121 GTAC GT^AC AflIII 731 ACATGT A^C[GA][CT]GT AgeI 548 ACCGGT A^CCGGT AlwNI 878 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 883 CCTGAGG CC^T[ACGT]AGG Aor51HI 335 AGCGCT AGC^GCT Asp700I 729 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 120 GGTACC G^GTACC BalI 796 TGGCCA TGG^CCA BanI 120 GGTACC G^G[CT][GA]CC BanII 67 GAGCCC G[GA]GC[CT]^C BbsI 255 GTCTTC GAAGAC(2/6) BbvCI 768 CCTCAGC CCTCAGC(-5/-2) BglI 184 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 255 GTCTTC GAAGAC(2/6) BsaI 59 GAGACC GGTCTC(1/5) BspLU11I 731 ACATGT A^CATGT BspMI 117 GCAGGT ACCTGC(4/8) BsrBI 301 GAGCGG CCGCTC(-3/-3) BsrFI 548 ACCGGT [GA]^CCGG[CT] BsrI 6 CCAGT ACTGG(1/-1) BstAPI 457 GCAATAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 297 GGATG GGATG(2/0) BstYI 540 GGATCT [GA]^GATC[CT] Bsu36I 883 CCTGAGG CC^T[ACGT]AGG Cfr10I 548 ACCGGT [GA]^CCGG[CT] DraII 879 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 796 TGGCCA [CT]^GGCC[GA] Eco47III 335 AGCGCT AGC^GCT Eco81I 883 CCTGAGG CC^T[ACGT]AGG EcoO109I 879 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 148 GATATC GAT^ATC FauI 303 GCGGG CCCGC(4/6) FokI 297 GGATG GGATG(9/13) KpnI 120 GGTACC GGTAC^C MaeII 208 ACGT A^CGT MflI 540 GGATCT [GA]^GATC[CT] MluNI 796 TGGCCA TGG^CCA MscI 796 TGGCCA TGG^CCA NspI 731 ACATGT [GA]CATG^[CT] PflMI 124 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 548 ACCGGT A^CCGGT PvuII 199 CAGCTG CAG^CTG RsaI 121 GTAC GT^AC SfcI 777 CTACAG C^T[GA][CT]AG SmaI 716 CCCGGG CCC^GGG SpeI 630 ACTAGT A^CTAGT Van91I 124 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 540 GGATCT [GA]^GATC[CT] XmaCI 716 CCCGGG C^CCGGG XmaI 716 CCCGGG C^CCGGG XmnI 729 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AciI 303 GCGG CCGC(-3/-1) 591 GCGG AluI 200 AGCT AG^CT 874 AGCT Alw44I 488 GTGCAC G^TGCAC 866 GTGCAC AlwI 540 GGATC GGATC(4/5) 701 GGATC ApaLI 488 GTGCAC G^TGCAC 866 GTGCAC AspHI 488 GTGCAC G[AT]GC[AT]^C 866 GTGCAC AvaII 164 GGACC G^G[AT]CC 663 GGACC BcnI 716 CCCGG CC^[GC]GG 717 CCGGG BfaI 500 CTAG C^TAG 631 CTAG BsaWI 548 ACCGGT [AT]^CCGG[AT] 654 TCCGGT BsgI 98 GTGCAG GTGCAG(16/14) 464 GTGCAG BsiHKAI 488 GTGCAC G[AT]GC[AT]^C 866 GTGCAC BsiYI 105 CCACCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 124 CCACCCTGTGG BslI 105 CCACCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 124 CCACCCTGTGG BsmAI 59 GAGAC GTCTC(1/5) 803 GTCTC BsrDI 615 CATTGC GCAATG(2/0) 344 GCAATG CfoI 288 GCGC GCG^C 336 GCGC DpnII 541 GATC ^GATC 702 GATC DraIII 15 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 125 CACCCTGTG HaeII 287 GGCGCT [GA]GCGC^[CT] 335 AGCGCT HhaI 288 GCGC GCG^C 336 GCGC HinP1I 288 GCGC G^CGC 336 GCGC HincII 157 GTCAAC GT[CT]^[GA]AC 358 GTTGAC HindII 157 GTCAAC GT[CT]^[GA]AC 358 GTTGAC MaeI 500 CTAG C^TAG 631 CTAG MboI 541 GATC ^GATC 702 GATC MseI 508 TTAA T^TAA 740 TTAA MslI 747 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 813 CACCTTTGTG MspA1I 199 CAGCTG C[AC]G^C[GT]G 589 CAGCGG NciI 716 CCCGG CC^[GC]GG 717 CCGGG NdeII 541 GATC ^GATC 702 GATC Sau3AI 541 GATC ^GATC 702 GATC StuI 191 AGGCCT AGG^CCT 791 AGGCCT TaqI 37 TCGA T^CGA 375 TCGA TfiI 27 GAATC G^A[AT]TC 482 GAATC Tru9I 508 TTAA T^TAA 740 TTAA TthHB8I 37 TCGA T^CGA 375 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AcsI ApoI AvaI BbvI BmyI BsaJI BseRI BsmFI BsoBI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboII MnlI MspI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI