Restriction map for
pFN21AE5428
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BciVI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 177 GTAC GT^AC
AlwI 471 GATCC GGATC(4/5)
AspHI 592 GTGCTC G[AT]GC[AT]^C
BbsI 307 GTCTTC GAAGAC(2/6)
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BcgI 537 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 158 CCCGG CC^[GC]GG
BfaI 224 CTAG C^TAG
BlpI 758 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 758 GCTCAGC GC^T[ACGT]AGC
BpuAI 307 GTCTTC GAAGAC(2/6)
BsaWI 512 ACCGGA [AT]^CCGG[AT]
BsgI 606 CTGCAC GTGCAG(16/14)
BsiHKAI 592 GTGCTC G[AT]GC[AT]^C
BsmAI 578 GTCTC GTCTC(1/5)
BspHI 404 TCATGA T^CATGA
BsrBI 684 CCGCTC CCGCTC(-3/-3)
BsrDI 660 CATTGC GCAATG(2/0)
BsrFI 380 GCCGGC [GA]^CCGG[CT]
CelII 758 GCTCAGC GC^T[ACGT]AGC
CfoI 896 GCGC GCG^C
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
DraIII 327 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 848 CCATGG C^C[GA][CT]GG
EaeI 785 CGGCCA [CT]^GGCC[GA]
EcoNI 531 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 146 CCCGC CCCGC(4/6)
HhaI 896 GCGC GCG^C
HinP1I 896 GCGC G^CGC
HincII 477 GTCAAC GT[CT]^[GA]AC
HindII 477 GTCAAC GT[CT]^[GA]AC
MaeI 224 CTAG C^TAG
MaeIII 841 GTCAC ^GT[ACGT]AC
MspA1I 64 CAGCTG C[AC]G^C[GT]G
NaeI 380 GCCGGC GCC^GGC
NciI 158 CCCGG CC^[GC]GG
NcoI 848 CCATGG C^CATGG
NgoMIV 380 GCCGGC G^CCGGC
PshAI 571 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 64 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
RsaI 177 GTAC GT^AC
SpeI 223 ACTAGT A^CTAGT
Tsp45I 841 GTCAC ^GT[GC]AC
Tsp509I 250 AATT ^AATT
XcmI 548 CCATGATCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BanI 509 GGCACC G^G[CT][GA]CC
540 GGTGCC
BclI 245 TGATCA T^GATCA
551 TGATCA
BmyI 508 GGGCAC G[AGT]GC[ACT]^C
592 GTGCTC
BpmI 67 CTGGAG CTGGAG(16/14)
151 CTGGAG
BsaAI 367 TACGTG [CT]AC^GT[GA]
717 TACGTG
BseRI 198 CTCCTC GAGGAG(10/8)
903 GAGGAG
Bsp1286I 508 GGGCAC G[AGT]GC[ACT]^C
592 GTGCTC
BsrI 558 ACTGG ACTGG(1/-1)
474 CCAGT
EcoT14I 203 CCTTGG C^C[AT][AT]GG
848 CCATGG
FbaI 245 TGATCA T^GATCA
551 TGATCA
HinfI 527 GATTC G^A[ACGT]TC
855 GAATC
MaeII 368 ACGT A^CGT
718 ACGT
PflMI 499 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCATCTGCTGG
SfaNI 335 GCATC GCATC(5/9)
635 GCATC
StyI 203 CCTTGG C^C[AT][AT]GG
848 CCATGG
TfiI 527 GATTC G^A[AT]TC
855 GAATC
Van91I 499 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCATCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
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AciI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI Ksp632I
MboI MboII MnlI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TseI TspRI