Restriction map for
pFN21AE2766
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII BstBI BstYI Bsu36I CelII
ClaI CpoI DpnII DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MboI MfeI MflI MluI MroI
MseI MunI NarI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SalI SapI
Sau3AI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 170 GTATAC GT^[AC][GT]AC
AlwNI 454 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 464 GGGCCC GGGCC^C
BalI 425 TGGCCA TGG^CCA
BbvI 757 GCTGC GCAGC(8/12)
BglI 119 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 552 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 695 TCATGA T^CATGA
BspMI 583 ACCTGC ACCTGC(4/8)
BsrBI 573 GAGCGG CCGCTC(-3/-3)
BsrFI 31 GCCGGC [GA]^CCGG[CT]
BssSI 469 CTCGTG CACGAG(-5/-1)
Bst1107I 170 GTATAC GTA^TAC
BstAPI 596 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 474 GGTCACC G^GT[ACGT]ACC
BstPI 474 GGTCACC G^GT[ACGT]ACC
BstXI 95 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 170 GTATAC GTA^TAC
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
DraIII 597 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
EcoO65I 474 GGTCACC G^GT[ACGT]ACC
KspI 219 CCGCGG CCGC^GG
MaeIII 475 GTCAC ^GT[ACGT]AC
MamI 552 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MslI 267 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 219 CCGCGG C[AC]G^C[GT]G
NaeI 31 GCCGGC GCC^GGC
NgoMIV 31 GCCGGC G^CCGGC
NspI 179 GCATGC [GA]CATG^[CT]
PflMI 453 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 695 TCATGA T^CATGA
SacII 219 CCGCGG CCGC^GG
SexAI 383 ACCTGGT A^CC[AT]GGT
SfaNI 257 GCATC GCATC(5/9)
SfcI 528 CTGTAG C^T[GA][CT]AG
SphI 179 GCATGC GCATG^C
TseI 757 GCTGC G^C[AT]GC
Tsp45I 475 GTCAC ^GT[GC]AC
Van91I 453 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 34 GGCGTC G[GA]^CG[CT]C
795 GACGCC
Alw44I 205 GTGCAC G^TGCAC
603 GTGCAC
ApaLI 205 GTGCAC G^TGCAC
603 GTGCAC
BanI 228 GGCACC G^G[CT][GA]CC
657 GGTGCC
BanII 464 GGGCCC G[GA]GC[CT]^C
744 GAGCCC
BsaHI 34 GGCGTC G[GA]^CG[CT]C
795 GACGCC
BseRI 634 GAGGAG GAGGAG(10/8)
709 GAGGAG
BsgI 594 CTGCAC GTGCAG(16/14)
758 CTGCAC
BsiEI 11 CGGCCG CG[GA][CT]^CG
216 CGACCG
BsrI 167 CCAGT ACTGG(1/-1)
210 CCAGT
DdeI 60 CTCAG C^T[ACGT]AG
666 CTTAG
EcoNI 524 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
705 CCTGGAGGAGG
Hin1I 34 GGCGTC G[GA]^CG[CT]C
795 GACGCC
MaeII 359 ACGT A^CGT
506 ACGT
PpuMI 406 GGGACCT [GA]G^G[AT]CC[CT]
700 AGGACCC
SmaI 402 CCCGGG CCC^GGG
739 CCCGGG
XmaCI 402 CCCGGG C^CCGGG
739 CCCGGG
XmaI 402 CCCGGG C^CCGGG
739 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BbsI BcnI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TfiI TspRI