Restriction map for
pFN21AE3110
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsmI
BsoBI Bsp1407I BspEI BspLU11I BsrBI
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AflIII 206 ACACGT A^C[GA][CT]GT
Alw44I 616 GTGCAC G^TGCAC
ApaLI 616 GTGCAC G^TGCAC
BamHI 266 GGATCC G^GATCC
BcgI 336 CGATGAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 730 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 18 TCCGGT [AT]^CCGG[AT]
BsmAI 601 GTCTC GTCTC(1/5)
BsmBI 600 CGTCTC CGTCTC(1/5)
BspDI 334 ATCGAT AT^CGAT
BspHI 701 TCATGA T^CATGA
BstXI 164 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 334 ATCGAT AT^CGAT
DdeI 260 CTCAG C^T[ACGT]AG
EarI 24 GAAGAG CTCTTC(1/4)
EcoNI 245 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 256 GGCGCT [GA]GCGC^[CT]
HindIII 631 AAGCTT A^AGCTT
Ksp632I 24 GAAGAG CTCTTC(1/4)
MslI 609 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 179 GCCGGC GCC^GGC
NgoMIV 179 GCCGGC G^CCGGC
NspI 342 GCATGC [GA]CATG^[CT]
PflMI 290 CCAACCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 701 TCATGA T^CATGA
SacI 196 GAGCTC GAGCT^C
SapI 24 GAAGAGC GCTCTTC(1/4)
SfiI 729 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 342 GCATGC GCATG^C
Tsp45I 646 GTCAC ^GT[GC]AC
TspRI 638 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 290 CCAACCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 161 CCACCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 126 GTAC GT^AC
429 GTAC
BalI 680 TGGCCA TGG^CCA
728 TGGCCA
BglII 545 AGATCT A^GATCT
836 AGATCT
BsiWI 125 CGTACG C^GTACG
428 CGTACG
BspMI 38 ACCTGC ACCTGC(4/8)
402 GCAGGT
BstF5I 165 CATCC GGATG(2/0)
786 CATCC
EagI 177 CGGCCG C^GGCCG
711 CGGCCG
EclXI 177 CGGCCG C^GGCCG
711 CGGCCG
Eco52I 177 CGGCCG C^GGCCG
711 CGGCCG
FokI 165 CATCC GGATG(9/13)
786 CATCC
MluNI 680 TGGCCA TGG^CCA
728 TGGCCA
MscI 680 TGGCCA TGG^CCA
728 TGGCCA
PstI 100 CTGCAG CTGCA^G
574 CTGCAG
RsaI 126 GTAC GT^AC
429 GTAC
SbfI 99 CCTGCAGG CCTGCA^GG
573 CCTGCAGG
Sse8387I 99 CCTGCAGG CCTGCA^GG
573 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI TthHB8I XhoII