Restriction map for
pFN21AE3677
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvrII BalI BbrPI BbsI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EarI Eco47III
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MboII
MfeI MluI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AhdI 466 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 790 CCTGAGG CC^T[ACGT]AGG
ApaI 57 GGGCCC GGGCC^C
AspEI 466 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 604 GGATCC G^GATCC
BbeI 339 GGCGCC GGCGC^C
BcgI 651 CGACCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 574 GTATCC GTATCC(6/5)
BfaI 418 CTAG C^TAG
BsaI 754 GAGACC GGTCTC(1/5)
BsrI 260 CCAGT ACTGG(1/-1)
BssSI 307 CACGAG CACGAG(-5/-1)
BstAPI 306 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 118 CCAGACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 604 GGATCC [GA]^GATC[CT]
Bsu36I 790 CCTGAGG CC^T[ACGT]AGG
Eam1105I 466 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 790 CCTGAGG CC^T[ACGT]AGG
EcoNI 423 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI 463 GGTGA GGTGA(8/7)
KasI 339 GGCGCC G^GCGCC
KspI 344 CCGCGG CCGC^GG
MaeI 418 CTAG C^TAG
MaeII 737 ACGT A^CGT
MaeIII 464 GTGAC ^GT[ACGT]AC
MflI 604 GGATCC [GA]^GATC[CT]
NaeI 452 GCCGGC GCC^GGC
NarI 339 GGCGCC GG^CGCC
NgoMIV 452 GCCGGC G^CCGGC
NspI 281 GCATGC [GA]CATG^[CT]
SacII 344 CCGCGG CCGC^GG
SfoI 339 GGCGCC GGC^GCC
SgrAI 445 CGCCGGTG C[GA]^CCGG[CT]G
SphI 281 GCATGC GCATG^C
StuI 420 AGGCCT AGG^CCT
TaqI 368 TCGA T^CGA
Tsp45I 464 GTGAC ^GT[GC]AC
TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 368 TCGA T^CGA
XhoII 604 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 339 GGCGCC G[GA]^CG[CT]C
594 GGCGTC
AluI 311 AGCT AG^CT
633 AGCT
AlwI 605 GATCC GGATC(4/5)
604 GGATC
AlwNI 7 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
352 CAGAGCCTG
AvaII 186 GGACC G^G[AT]CC
473 GGTCC
AviII 278 TGCGCA TGC^GCA
284 TGCGCA
BbvCI 423 CCTCAGC CCTCAGC(-5/-2)
538 GCTGAGG
BglI 230 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
670 GCCGAGGCGGC
BpmI 439 CTCCAG CTGGAG(16/14)
778 CTGGAG
BsaHI 339 GGCGCC G[GA]^CG[CT]C
594 GGCGTC
BseRI 430 GAGGAG GAGGAG(10/8)
793 GAGGAG
BsgI 304 CTGCAC GTGCAG(16/14)
635 CTGCAC
BsmBI 108 CGTCTC CGTCTC(1/5)
370 GAGACG
BsrFI 446 GCCGGT [GA]^CCGG[CT]
452 GCCGGC
BssHII 218 GCGCGC G^CGCGC
220 GCGCGC
BstF5I 38 GGATG GGATG(2/0)
761 GGATG
Cfr10I 446 GCCGGT [GA]^CCGG[CT]
452 GCCGGC
DpnII 366 GATC ^GATC
605 GATC
FokI 38 GGATG GGATG(9/13)
761 GGATG
FspI 278 TGCGCA TGC^GCA
284 TGCGCA
Hin1I 339 GGCGCC G[GA]^CG[CT]C
594 GGCGTC
MboI 366 GATC ^GATC
605 GATC
NdeII 366 GATC ^GATC
605 GATC
NlaIII 125 CATG CATG^
282 CATG
NotI 69 GCGGCCGC GC^GGCCGC
95 GCGGCCGC
PpuMI 185 GGGACCC [GA]G^G[AT]CC[CT]
472 GGGTCCC
Sau3AI 366 GATC ^GATC
605 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AvaI BanI BanII
BbvI BcnI BmyI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV PleI Sau96I ScrFI SfaNI
TseI