Restriction map for
pFN21AE5130
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HindIII
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 314 AAATTC [GA]^AATT[CT]
AfaI 274 GTAC GT^AC
Alw44I 224 GTGCAC G^TGCAC
AlwI 99 GATCC GGATC(4/5)
ApaLI 224 GTGCAC G^TGCAC
ApoI 314 AAATTC [GA]^AATT[CT]
AvaI 18 CCCGAG C^[CT]CG[GA]G
BanI 54 GGTGCC G^G[CT][GA]CC
BbsI 376 GAAGAC GAAGAC(2/6)
BlpI 36 GCTGAGC GC^T[ACGT]AGC
BpmI 103 CTGGAG CTGGAG(16/14)
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
BpuAI 376 GAAGAC GAAGAC(2/6)
BsaI 264 GAGACC GGTCTC(1/5)
BsaWI 429 TCCGGT [AT]^CCGG[AT]
BsiEI 231 CGGCCG CG[GA][CT]^CG
BsmAI 264 GAGAC GTCTC(1/5)
BsmFI 213 GGGAC GGGAC(10/14)
BsoBI 18 CCCGAG C^[CT]CG[GA]G
BsrFI 251 GCCGGC [GA]^CCGG[CT]
BsrI 443 CCAGT ACTGG(1/-1)
CelII 36 GCTGAGC GC^T[ACGT]AGC
CfoI 255 GCGC GCG^C
Cfr10I 251 GCCGGC [GA]^CCGG[CT]
DpnII 99 GATC ^GATC
DraIII 218 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 231 CGGCCG [CT]^GGCC[GA]
EagI 231 CGGCCG C^GGCCG
EclXI 231 CGGCCG C^GGCCG
Eco52I 231 CGGCCG C^GGCCG
HaeII 254 GGCGCT [GA]GCGC^[CT]
HhaI 255 GCGC GCG^C
HinP1I 255 GCGC G^CGC
HincII 294 GTTAAC GT[CT]^[GA]AC
HindII 294 GTTAAC GT[CT]^[GA]AC
HpaI 294 GTTAAC GTT^AAC
MboI 99 GATC ^GATC
MseI 295 TTAA T^TAA
MslI 90 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 251 GCCGGC GCC^GGC
NdeII 99 GATC ^GATC
NgoMIV 251 GCCGGC G^CCGGC
RsaI 274 GTAC GT^AC
Sau3AI 99 GATC ^GATC
SfaNI 336 GCATC GCATC(5/9)
SmlI 59 CTCAAG C^T[CT][GA]AG
Tru9I 295 TTAA T^TAA
Tsp509I 315 AATT ^AATT
XcmI 92 CCACCATGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AvaII 48 GGTCC G^G[AT]CC
214 GGACC
BcgI 241 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
330 GCAAGAGCATCG
BstXI 95 CCATGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
171 CCAGAGTGCTGG
EcoT14I 44 CCTTGG C^C[AT][AT]GG
281 CCAAGG
HapII 252 CCGG C^CGG
430 CCGG
HinfI 112 GACTC G^A[ACGT]TC
154 GAGTC
HpaII 252 CCGG C^CGG
430 CCGG
MboII 67 GAAGA GAAGA(8/7)
376 GAAGA
MspA1I 34 CCGCTG C[AC]G^C[GT]G
362 CAGCGG
MspI 252 CCGG C^CGG
430 CCGG
NlaIII 96 CATG CATG^
300 CATG
PleI 112 GACTC GAGTC(4/5)
154 GAGTC
PstI 8 CTGCAG CTGCA^G
136 CTGCAG
SacI 39 GAGCTC GAGCT^C
244 GAGCTC
StyI 44 CCTTGG C^C[AT][AT]GG
281 CCAAGG
TspRI 351 CAGTG CA[GC]TG[ACGT][ACGT]^
402 CAGTG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstEII BstF5I
BstNI BstPI Cac8I Cfr13I DdeI
EcoO65I EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeIII MnlI MvaI
MwoI NlaIV Sau96I ScrFI SfcI
TseI Tsp45I