##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsoBI       Bsp1286I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AlwI          612  GATCC                GGATC(4/5)
Asp700I       106  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsmAI         411  GTCTC                GTCTC(1/5)
BsmFI         468  GGGAC                GGGAC(10/14)
BsmI          484  GAATGC               GAATGC(1/-1)
BspMI         150  ACCTGC               ACCTGC(4/8)
BsrFI         488  GCCGGT               [GA]^CCGG[CT]
BstYI         611  AGATCC               [GA]^GATC[CT]
Cac8I         360  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr10I        488  GCCGGT               [GA]^CCGG[CT]
DpnII         612  GATC                 ^GATC
EarI          397  GAAGAG               CTCTTC(1/4)
HapII         489  CCGG                 C^CGG
HpaII         489  CCGG                 C^CGG
HphI          604  GGTGA                GGTGA(8/7)
Ksp632I       397  GAAGAG               CTCTTC(1/4)
MboI          612  GATC                 ^GATC
MflI          611  AGATCC               [GA]^GATC[CT]
MspI          489  CCGG                 C^CGG
NdeII         612  GATC                 ^GATC
NspI           83  GCATGT               [GA]CATG^[CT]
PleI          235  GACTC                GAGTC(4/5)
SapI          397  GAAGAGC              GCTCTTC(1/4)
Sau3AI        612  GATC                 ^GATC
SfcI          296  CTGTAG               C^T[GA][CT]AG
SmlI            5  CTCAAG               C^T[CT][GA]AG
Tsp45I        279  GTCAC                ^GT[GC]AC
XhoII         611  AGATCC               [GA]^GATC[CT]
XmnI          106  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AlwNI         290  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              338  CAGTACCTG           
BsgI          358  GTGCAG               GTGCAG(16/14)
              453  GTGCAG              
Bsp1407I      513  TGTACA               T^GTACA
              531  TGTACA              
BsrGI         513  TGTACA               T^GTACA
              531  TGTACA              
DdeI          525  CTCAG                C^T[ACGT]AG
              569  CTGAG               
MaeIII        279  GTCAC                ^GT[ACGT]AC
              521  GTTAC               
MfeI          310  CAATTG               C^AATTG
              537  CAATTG              
MnlI           70  CCTC                 CCTC(7/6)
              158  CCTC                
MunI          310  CAATTG               C^AATTG
              537  CAATTG              
NlaIII         84  CATG                 CATG^
              450  CATG                
SfaNI         562  GATGC                GCATC(5/9)
              301  GCATC               
SspBI         513  TGTACA               T^GTACA
              531  TGTACA              
TspRI          45  CACTG                CA[GC]TG[ACGT][ACGT]^
              207  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AfaI        BbvI        BsrI        Fnu4HI      HinfI       
ItaI        MboII       MwoI        RsaI        TfiI        
TseI        Tsp509I