Restriction map for
pFN21AE3156
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HhaI HinP1I HpaI HpaII KasI
KpnI KspI MamI MluI MluNI
MroI MscI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI Tth111I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 534 AAATTT [GA]^AATT[CT]
AcyI 39 GGCGTC G[GA]^CG[CT]C
AlwI 841 GATCC GGATC(4/5)
ApoI 534 AAATTT [GA]^AATT[CT]
AspHI 634 GAGCTC G[AT]GC[AT]^C
BanI 117 GGTGCC G^G[CT][GA]CC
BglII 770 AGATCT A^GATCT
BpmI 936 CTGGAG CTGGAG(16/14)
BsaHI 39 GGCGTC G[GA]^CG[CT]C
BsaJI 377 CCACGG C^C[ACGT][ACGT]GG
BseRI 899 GAGGAG GAGGAG(10/8)
BsiHKAI 634 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1074 TGTACA T^GTACA
BsrGI 1074 TGTACA T^GTACA
BssSI 61 CACGAG CACGAG(-5/-1)
BstAPI 138 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 579 CCATTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 770 AGATCT [GA]^GATC[CT]
DraI 582 TTTAAA TTT^AAA
DsaI 377 CCACGG C^C[GA][CT]GG
FauI 135 CCCGC CCCGC(4/6)
HaeIII 21 GGCC GG^CC
HgaI 40 GCGTC GACGC(5/10)
Hin1I 39 GGCGTC G[GA]^CG[CT]C
HincII 736 GTTGAC GT[CT]^[GA]AC
HindII 736 GTTGAC GT[CT]^[GA]AC
HindIII 611 AAGCTT A^AGCTT
HphI 199 TCACC GGTGA(8/7)
MaeII 252 ACGT A^CGT
MfeI 292 CAATTG C^AATTG
MflI 770 AGATCT [GA]^GATC[CT]
MunI 292 CAATTG C^AATTG
NlaIV 117 GGTGCC GG[ACGT]^[ACGT]CC
NspI 717 GCATGT [GA]CATG^[CT]
PflMI 108 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 564 GACTC GAGTC(4/5)
SacI 634 GAGCTC GAGCT^C
SapI 484 GAAGAGC GCTCTTC(1/4)
SmlI 16 CTCAAG C^T[CT][GA]AG
SspBI 1074 TGTACA T^GTACA
SspI 543 AATATT AAT^ATT
SwaI 581 ATTTAAAT ATTT^AAAT
Tsp45I 406 GTGAC ^GT[GC]AC
Van91I 108 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 255 TCTAGA T^CTAGA
XhoII 770 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 1075 GTAC GT^AC
1101 GTAC
AlwNI 109 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
593 CAGTGTCTG
BsmFI 391 GGGAC GGGAC(10/14)
641 GGGAC
BsrI 713 ACTGG ACTGG(1/-1)
108 CCAGT
BstF5I 348 GGATG GGATG(2/0)
513 GGATG
Cac8I 134 GCCCGC GC[ACGT]^[ACGT]GC
609 GCAAGC
FokI 348 GGATG GGATG(9/13)
513 GGATG
MaeIII 406 GTGAC ^GT[ACGT]AC
864 GTTAC
PstI 318 CTGCAG CTGCA^G
727 CTGCAG
RsaI 1075 GTAC GT^AC
1101 GTAC
SfaNI 357 GATGC GCATC(5/9)
74 GCATC
SfcI 318 CTGCAG C^T[GA][CT]AG
727 CTGCAG
TaqI 50 TCGA T^CGA
855 TCGA
TthHB8I 50 TCGA T^CGA
855 TCGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BanII BbsI BbvI
BfaI BmyI BpuAI BsgI BsiYI
BslI Bsp1286I BssKI BstNI DdeI
DpnII EarI EcoRII Fnu4HI HinfI
ItaI Ksp632I MaeI MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI TfiI
Tru9I TseI Tsp509I TspRI