Restriction map for
pFN21AE5077
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflIII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BalI BbvCI BcgI BciVI BclI
BglII BlpI Bpu1102I BsaBI BsaI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
KspI MamI MfeI MluI MluNI
MscI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 137 TCCGGA T^CCGGA
AflII 385 CTTAAG C^TTAAG
AluI 531 AGCT AG^CT
Alw44I 106 GTGCAC G^TGCAC
AlwNI 201 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 106 GTGCAC G^TGCAC
AspHI 106 GTGCAC G[AT]GC[AT]^C
AvrII 516 CCTAGG C^CTAGG
BamHI 432 GGATCC G^GATCC
BanII 69 GGGCTC G[GA]GC[CT]^C
BbrPI 234 CACGTG CAC^GTG
BbsI 196 GTCTTC GAAGAC(2/6)
BfrI 385 CTTAAG C^TTAAG
BlnI 516 CCTAGG C^CTAGG
BpmI 533 CTGGAG CTGGAG(16/14)
BpuAI 196 GTCTTC GAAGAC(2/6)
BsaAI 234 CACGTG [CT]AC^GT[GA]
BseAI 137 TCCGGA T^CCGGA
BseRI 510 GAGGAG GAGGAG(10/8)
BsgI 271 CTGCAC GTGCAG(16/14)
BsiHKAI 106 GTGCAC G[AT]GC[AT]^C
BsiWI 303 CGTACG C^GTACG
BsmAI 374 GTCTC GTCTC(1/5)
BspEI 137 TCCGGA T^CCGGA
BsrFI 363 GCCGGC [GA]^CCGG[CT]
BssHII 186 GCGCGC G^CGCGC
Cfr10I 363 GCCGGC [GA]^CCGG[CT]
DdeI 376 CTCAG C^T[ACGT]AG
EarI 28 GAAGAG CTCTTC(1/4)
EcoNI 270 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 462 GAATC G^A[ACGT]TC
Ksp632I 28 GAAGAG CTCTTC(1/4)
MroI 137 TCCGGA T^CCGGA
MseI 386 TTAA T^TAA
NaeI 363 GCCGGC GCC^GGC
NgoMIV 363 GCCGGC G^CCGGC
NspI 110 ACATGC [GA]CATG^[CT]
PmaCI 234 CACGTG CAC^GTG
PmlI 234 CACGTG CAC^GTG
PvuII 530 CAGCTG CAG^CTG
SexAI 275 ACCAGGT A^CC[AT]GGT
SmaI 66 CCCGGG CCC^GGG
TfiI 462 GAATC G^A[AT]TC
Tru9I 386 TTAA T^TAA
XmaCI 66 CCCGGG C^CCGGG
XmaI 66 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 304 GTAC GT^AC
382 GTAC
AvaI 66 CCCGGG C^[CT]CG[GA]G
562 CCCGAG
AvaII 335 GGTCC G^G[AT]CC
475 GGTCC
BanI 33 GGCGCC G^G[CT][GA]CC
93 GGCGCC
BbeI 33 GGCGCC GGCGC^C
93 GGCGCC
BfaI 371 CTAG C^TAG
517 CTAG
BglI 163 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
260 GCCTGGCGGGC
BsaWI 137 TCCGGA [AT]^CCGG[AT]
296 ACCGGA
BsiEI 210 CGGCCG CG[GA][CT]^CG
365 CGGCCG
BsmFI 476 GTCCC GGGAC(10/14)
483 GTCCC
BsoBI 66 CCCGGG C^[CT]CG[GA]G
562 CCCGAG
BstF5I 408 CATCC GGATG(2/0)
469 CATCC
EaeI 210 CGGCCG [CT]^GGCC[GA]
365 CGGCCG
EagI 210 CGGCCG C^GGCCG
365 CGGCCG
EclXI 210 CGGCCG C^GGCCG
365 CGGCCG
Eco52I 210 CGGCCG C^GGCCG
365 CGGCCG
EcoT14I 419 CCAAGG C^C[AT][AT]GG
516 CCTAGG
FokI 408 CATCC GGATG(9/13)
469 CATCC
KasI 33 GGCGCC G^GCGCC
93 GGCGCC
MaeI 371 CTAG C^TAG
517 CTAG
MaeII 235 ACGT A^CGT
392 ACGT
MaeIII 232 GTCAC ^GT[ACGT]AC
280 GTCAC
MboII 28 GAAGA GAAGA(8/7)
197 TCTTC
NarI 33 GGCGCC GG^CGCC
93 GGCGCC
NlaIII 111 CATG CATG^
168 CATG
RsaI 304 GTAC GT^AC
382 GTAC
SfoI 33 GGCGCC GGC^GCC
93 GGCGCC
SmlI 250 CTCAAG C^T[CT][GA]AG
385 CTTAAG
StyI 419 CCAAGG C^C[AT][AT]GG
516 CCTAGG
Tsp45I 232 GTCAC ^GT[GC]AC
280 GTCAC
XcmI 158 CCACCGCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
419 CCAAGGACGCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AcyI AlwI BbvI
BcnI BmyI BsaHI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI MboI MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI TseI XhoII