Restriction map for
pFN21AE3764
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AspI AviII AvrII BbeI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI HinP1I HincII HindII
HindIII HpaI KasI KspI MaeI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
SfoI SfuI SgrAI SmlI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 985 GACGTC GACGT^C
Acc65I 328 GGTACC G^GTACC
AccII 10 CGCG CG^CG
AccIII 136 TCCGGA T^CCGGA
AcsI 1130 AAATTC [GA]^AATT[CT]
AcyI 985 GACGTC G[GA]^CG[CT]C
AflIII 1358 ACACGT A^C[GA][CT]GT
Alw44I 249 GTGCAC G^TGCAC
ApaLI 249 GTGCAC G^TGCAC
ApoI 1130 AAATTC [GA]^AATT[CT]
AseI 1396 ATTAAT AT^TAAT
AsnI 1396 ATTAAT AT^TAAT
Asp700I 1492 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 328 GGTACC G^GTACC
AspHI 249 GTGCAC G[AT]GC[AT]^C
BbrPI 1359 CACGTG CAC^GTG
BglI 286 GCCAACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1590 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1590 GCTGAGC GC^T[ACGT]AGC
BsaBI 1858 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 985 GACGTC G[GA]^CG[CT]C
BseAI 136 TCCGGA T^CCGGA
BsiEI 146 CGGTCG CG[GA][CT]^CG
BsiHKAI 249 GTGCAC G[AT]GC[AT]^C
BsmAI 809 GTCTC GTCTC(1/5)
BspEI 136 TCCGGA T^CCGGA
BspHI 1113 TCATGA T^CATGA
BspMI 197 ACCTGC ACCTGC(4/8)
BssSI 34 CTCGTG CACGAG(-5/-1)
BstUI 10 CGCG CG^CG
CelII 1590 GCTGAGC GC^T[ACGT]AGC
Hin1I 985 GACGTC G[GA]^CG[CT]C
KpnI 328 GGTACC GGTAC^C
MamI 1858 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 136 TCCGGA T^CCGGA
MslI 813 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 10 CGCG CG^CG
NcoI 1468 CCATGG C^CATGG
NspI 802 ACATGC [GA]CATG^[CT]
PmaCI 1359 CACGTG CAC^GTG
PmlI 1359 CACGTG CAC^GTG
PshBI 1396 ATTAAT AT^TAAT
PvuII 1558 CAGCTG CAG^CTG
RcaI 1113 TCATGA T^CATGA
SapI 1408 GCTCTTC GCTCTTC(1/4)
ScaI 1031 AGTACT AGT^ACT
SexAI 446 ACCTGGT A^CC[AT]GGT
SfiI 285 GGCCAACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 916 TACGTA TAC^GTA
SspI 1118 AATATT AAT^ATT
Tsp45I 950 GTCAC ^GT[GC]AC
Tsp509I 1131 AATT ^AATT
XcmI 1190 CCACGGAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1492 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 64 CCCGGG C^[CT]CG[GA]G
688 CCCGGG
BalI 284 TGGCCA TGG^CCA
1665 TGGCCA
BamHI 888 GGATCC G^GATCC
1722 GGATCC
BsaAI 916 TACGTA [CT]AC^GT[GA]
1359 CACGTG
BsaWI 136 TCCGGA [AT]^CCGG[AT]
1019 TCCGGT
BsoBI 64 CCCGGG C^[CT]CG[GA]G
688 CCCGGG
DraII 368 GGGACCC [GA]G^G[ACGT]CC[CT]
443 AGGACCT
DsaI 1190 CCACGG C^C[GA][CT]GG
1468 CCATGG
EcoO109I 368 GGGACCC [GA]G^G[ACGT]CC[CT]
443 AGGACCT
HphI 1737 GGTGA GGTGA(8/7)
195 TCACC
MaeIII 649 GTTAC ^GT[ACGT]AC
950 GTCAC
MluNI 284 TGGCCA TGG^CCA
1665 TGGCCA
MscI 284 TGGCCA TGG^CCA
1665 TGGCCA
MspA1I 381 CAGCGG C[AC]G^C[GT]G
1558 CAGCTG
NdeI 856 CATATG CA^TATG
1789 CATATG
PflMI 1150 CCAGTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1452 CCAACCACTGG
PpuMI 368 GGGACCC [GA]G^G[AT]CC[CT]
443 AGGACCT
SfaNI 69 GATGC GCATC(5/9)
211 GATGC
SmaI 64 CCCGGG CCC^GGG
688 CCCGGG
TaqI 109 TCGA T^CGA
1644 TCGA
TfiI 481 GATTC G^A[AT]TC
822 GAATC
TthHB8I 109 TCGA T^CGA
1644 TCGA
Van91I 1150 CCAGTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1452 CCAACCACTGG
XmaCI 64 CCCGGG C^CCGGG
688 CCCGGG
XmaI 64 CCCGGG C^CCGGG
688 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI StyI Tru9I TseI TspRI
XhoII