Restriction map for
pFN21AE2385
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 15 CGCG CG^CG
AlwNI 1572 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1681 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1665 GGATCC G^GATCC
BbvCI 338 CCTCAGC CCTCAGC(-5/-2)
BcgI 1803 GCACCCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1410 GGATAC GTATCC(6/5)
BglI 1111 GCCAATCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 195 GTGCCC G[AGT]GC[ACT]^C
BsaBI 1549 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 105 GAGACC GGTCTC(1/5)
BseRI 1600 CTCCTC GAGGAG(10/8)
BsgI 808 CTGCAC GTGCAG(16/14)
BsmI 1752 GCATTC GAATGC(1/-1)
Bsp1286I 195 GTGCCC G[AGT]GC[ACT]^C
BsrBI 1597 CCGCTC CCGCTC(-3/-3)
BsrDI 1491 CATTGC GCAATG(2/0)
BstAPI 1237 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1533 GGTGACC G^GT[ACGT]ACC
BstPI 1533 GGTGACC G^GT[ACGT]ACC
BstUI 15 CGCG CG^CG
BstYI 1665 GGATCC [GA]^GATC[CT]
Cac8I 830 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 14 GCGC GCG^C
DraIII 1369 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 482 CCTACGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1533 GGTGACC G^GT[ACGT]ACC
EcoT14I 308 CCAAGG C^C[AT][AT]GG
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
MaeII 1150 ACGT A^CGT
MamI 1549 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1665 GGATCC [GA]^GATC[CT]
MvnI 15 CGCG CG^CG
PflFI 1681 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 1645 ACCAGGT A^CC[AT]GGT
SfcI 119 CTACAG C^T[GA][CT]AG
SspI 945 AATATT AAT^ATT
StyI 308 CCAAGG C^C[AT][AT]GG
SwaI 890 ATTTAAAT ATTT^AAAT
TaqI 1226 TCGA T^CGA
Tth111I 1681 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1226 TCGA T^CGA
XhoII 1665 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflIII 1540 ACATGT A^C[GA][CT]GT
1851 ACATGT
BpmI 36 CTCCAG CTGGAG(16/14)
1826 CTGGAG
BspLU11I 1540 ACATGT A^CATGT
1851 ACATGT
DpnII 670 GATC ^GATC
1666 GATC
DraI 796 TTTAAA TTT^AAA
891 TTTAAA
EcoRI 219 GAATTC G^AATTC
1440 GAATTC
FauI 1108 CCCGC CCCGC(4/6)
1841 GCGGG
HapII 1117 CCGG C^CGG
1400 CCGG
HpaII 1117 CCGG C^CGG
1400 CCGG
MboI 670 GATC ^GATC
1666 GATC
MslI 1267 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1417 CATATTTATG
MspI 1117 CCGG C^CGG
1400 CCGG
NdeII 670 GATC ^GATC
1666 GATC
NspI 1540 ACATGT [GA]CATG^[CT]
1851 ACATGT
PvuII 341 CAGCTG CAG^CTG
1575 CAGCTG
Sau3AI 670 GATC ^GATC
1666 GATC
XcmI 449 CCACACCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1331 CCAGTTCTTCTATGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbsI BbvI BfaI BpuAI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cfr13I DdeI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboII MnlI MseI MspA1I MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI