Restriction map for
pFN21AE9687
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FokI
FseI HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MunI
NarI NdeI NdeII NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 293 CGCG CG^CG
AfaI 389 GTAC GT^AC
Alw44I 271 GTGCAC G^TGCAC
AlwNI 10 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 271 GTGCAC G^TGCAC
AviII 238 TGCGCA TGC^GCA
BbsI 313 GTCTTC GAAGAC(2/6)
BbvCI 169 GCTGAGG CCTCAGC(-5/-2)
BciVI 117 GGATAC GTATCC(6/5)
BfaI 358 CTAG C^TAG
BlpI 7 GCTCAGC GC^T[ACGT]AGC
BpmI 318 CTCCAG CTGGAG(16/14)
Bpu1102I 7 GCTCAGC GC^T[ACGT]AGC
BpuAI 313 GTCTTC GAAGAC(2/6)
BseRI 172 GAGGAG GAGGAG(10/8)
BspMI 123 GCAGGT ACCTGC(4/8)
BsrBI 145 CCGCTC CCGCTC(-3/-3)
BsrFI 184 GCCGGC [GA]^CCGG[CT]
BstUI 293 CGCG CG^CG
CelII 7 GCTCAGC GC^T[ACGT]AGC
Cfr10I 184 GCCGGC [GA]^CCGG[CT]
EcoT14I 107 CCATGG C^C[AT][AT]GG
EcoT22I 542 ATGCAT ATGCA^T
FspI 238 TGCGCA TGC^GCA
HaeII 348 AGCGCC [GA]GCGC^[CT]
HindIII 89 AAGCTT A^AGCTT
HphI 129 GGTGA GGTGA(8/7)
MaeI 358 CTAG C^TAG
MvnI 293 CGCG CG^CG
NaeI 184 GCCGGC GCC^GGC
NcoI 107 CCATGG C^CATGG
NgoMIV 184 GCCGGC G^CCGGC
NsiI 542 ATGCAT ATGCA^T
PflMI 107 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 208 CTGCAG CTGCA^G
PvuII 193 CAGCTG CAG^CTG
RsaI 389 GTAC GT^AC
ScaI 388 AGTACT AGT^ACT
SfcI 208 CTGCAG C^T[GA][CT]AG
SmaI 47 CCCGGG CCC^GGG
SmlI 471 CTTGAG C^T[CT][GA]AG
StyI 107 CCATGG C^C[AT][AT]GG
Van91I 107 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 494 CCAGTGACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 47 CCCGGG C^CCGGG
XmaI 47 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 256 GTGCTC G[AT]GC[AT]^C
271 GTGCAC
AvaI 47 CCCGGG C^[CT]CG[GA]G
301 CTCGGG
BcnI 47 CCCGG CC^[GC]GG
48 CCGGG
BglI 12 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
198 GCCGTCTTGGC
BmyI 256 GTGCTC G[AGT]GC[ACT]^C
271 GTGCAC
BsiHKAI 256 GTGCTC G[AT]GC[AT]^C
271 GTGCAC
BsoBI 47 CCCGGG C^[CT]CG[GA]G
301 CTCGGG
Bsp1286I 256 GTGCTC G[AGT]GC[ACT]^C
271 GTGCAC
BstNI 281 CCTGG CC^[AT]GG
462 CCTGG
DraII 136 GGGTCCT [GA]G^G[ACGT]CC[CT]
284 GGGACCT
DraIII 29 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
250 CACGCAGTG
DsaI 107 CCATGG C^C[GA][CT]GG
308 CCGTGG
EcoO109I 136 GGGTCCT [GA]G^G[ACGT]CC[CT]
284 GGGACCT
EcoRII 281 CCTGG ^CC[AT]GG
462 CCTGG
FauI 154 CCCGC CCCGC(4/6)
189 CCCGC
HapII 48 CCGG C^CGG
185 CCGG
HpaII 48 CCGG C^CGG
185 CCGG
MaeII 30 ACGT A^CGT
335 ACGT
MseI 520 TTAA T^TAA
628 TTAA
MspA1I 13 CCGCTG C[AC]G^C[GT]G
193 CAGCTG
MspI 48 CCGG C^CGG
185 CCGG
MvaI 281 CCTGG CC^[AT]GG
462 CCTGG
NciI 47 CCCGG CC^[GC]GG
48 CCGGG
NlaIII 108 CATG CATG^
454 CATG
PleI 265 GACTC GAGTC(4/5)
660 GACTC
PpuMI 136 GGGTCCT [GA]G^G[AT]CC[CT]
284 GGGACCT
SfaNI 21 GCATC GCATC(5/9)
544 GCATC
TfiI 423 GATTC G^A[AT]TC
645 GATTC
Tru9I 520 TTAA T^TAA
628 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI BsrI BssKI
Cac8I CfoI Cfr13I DdeI Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MaeIII MboII MnlI MwoI NlaIV
Sau96I ScrFI TseI Tsp45I TspRI