Restriction map for
pFN21AE3045
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AgeI AhdI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BalI BamHI BbeI BbsI
BbvCI BciVI BclI BfaI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeI MamI
MboI MfeI MflI MluI MluNI
MscI MspA1I MunI MvnI NarI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 392 TCCGGA T^CCGGA
AcsI 442 AAATTC [GA]^AATT[CT]
AflII 295 CTTAAG C^TTAAG
ApoI 442 AAATTC [GA]^AATT[CT]
AspI 138 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 205 CCCGGG C^[CT]CG[GA]G
BanI 91 GGTGCC G^G[CT][GA]CC
BanII 26 GAGCCC G[GA]GC[CT]^C
BbrPI 512 CACGTG CAC^GTG
BfrI 295 CTTAAG C^TTAAG
BsaAI 512 CACGTG [CT]AC^GT[GA]
BsaWI 392 TCCGGA [AT]^CCGG[AT]
BseAI 392 TCCGGA T^CCGGA
BsmAI 272 GAGAC GTCTC(1/5)
BsoBI 205 CCCGGG C^[CT]CG[GA]G
BspDI 541 ATCGAT AT^CGAT
BspEI 392 TCCGGA T^CCGGA
BsrFI 57 GCCGGC [GA]^CCGG[CT]
BssSI 310 CACGAG CACGAG(-5/-1)
CfoI 255 GCGC GCG^C
Cfr10I 57 GCCGGC [GA]^CCGG[CT]
ClaI 541 ATCGAT AT^CGAT
EaeI 357 CGGCCA [CT]^GGCC[GA]
EarI 501 GAAGAG CTCTTC(1/4)
HhaI 255 GCGC GCG^C
HinP1I 255 GCGC G^CGC
Ksp632I 501 GAAGAG CTCTTC(1/4)
MaeII 513 ACGT A^CGT
MroI 392 TCCGGA T^CCGGA
MseI 296 TTAA T^TAA
NaeI 57 GCCGGC GCC^GGC
NgoMIV 57 GCCGGC G^CCGGC
NspI 243 GCATGT [GA]CATG^[CT]
PflFI 138 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 512 CACGTG CAC^GTG
PmlI 512 CACGTG CAC^GTG
SfcI 481 CTACAG C^T[GA][CT]AG
SmaI 205 CCCGGG CCC^GGG
SmlI 295 CTTAAG C^T[CT][GA]AG
TaqI 542 TCGA T^CGA
Tru9I 296 TTAA T^TAA
Tsp509I 443 AATT ^AATT
Tth111I 138 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 542 TCGA T^CGA
XmaCI 205 CCCGGG C^CCGGG
XmaI 205 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 511 ACACGT A^C[GA][CT]GT
513 ACGTGT
AvaII 47 GGACC G^G[AT]CC
83 GGTCC
BcgI 271 CGAGACTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
533 GCATATCCATCG
BmyI 26 GAGCCC G[AGT]GC[ACT]^C
192 GGGCAC
BpmI 162 CTCCAG CTGGAG(16/14)
220 CTGGAG
BseRI 37 CTCCTC GAGGAG(10/8)
211 GAGGAG
BsmFI 46 GGGAC GGGAC(10/14)
84 GTCCC
Bsp1286I 26 GAGCCC G[AGT]GC[ACT]^C
192 GGGCAC
BsrI 275 ACTGG ACTGG(1/-1)
360 CCAGT
BstXI 150 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
539 CCATCGATTTGG
Cac8I 57 GCCGGC GC[ACGT]^[ACGT]GC
431 GCAAGC
DdeI 129 CTCAG C^T[ACGT]AG
140 CTCAG
EcoT14I 87 CCAAGG C^C[AT][AT]GG
183 CCAAGG
MboII 498 GAAGA GAAGA(8/7)
501 GAAGA
PpuMI 46 GGGACCC [GA]G^G[AT]CC[CT]
82 GGGTCCC
StyI 87 CCAAGG C^C[AT][AT]GG
183 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BbvI BcnI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeIII MnlI MslI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI Sau96I ScrFI TseI Tsp45I
TspRI