Restriction map for
pFN21AE1165
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BamHI BbrPI BbvCI BciVI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoT22I FseI FspI
KpnI KspI MaeI MaeII MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 793 CGCG CG^CG
AccIII 158 TCCGGA T^CCGGA
AfaI 648 GTAC GT^AC
AlwNI 50 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1207 AGCGCT AGC^GCT
ApaI 418 GGGCCC GGGCC^C
AspI 1686 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1388 TGGCCA TGG^CCA
BanI 117 GGCGCC G^G[CT][GA]CC
BanII 418 GGGCCC G[GA]GC[CT]^C
BbeI 117 GGCGCC GGCGC^C
BcgI 900 GCAGGATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 848 TGATCA T^GATCA
BcnI 1455 CCGGG CC^[GC]GG
BglI 1051 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1135 GCTCAGC GC^T[ACGT]AGC
BmyI 418 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 1135 GCTCAGC GC^T[ACGT]AGC
BsaWI 158 TCCGGA [AT]^CCGG[AT]
BseAI 158 TCCGGA T^CCGGA
Bsp1286I 418 GGGCCC G[AGT]GC[ACT]^C
BspEI 158 TCCGGA T^CCGGA
BsrDI 1106 CATTGC GCAATG(2/0)
BsrFI 1078 GCCGGC [GA]^CCGG[CT]
BssSI 1073 CTCGTG CACGAG(-5/-1)
BstUI 793 CGCG CG^CG
CelII 1135 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1078 GCCGGC [GA]^CCGG[CT]
DraI 1311 TTTAAA TTT^AAA
DrdI 1261 GACAACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 452 CCATGG C^C[GA][CT]GG
EaeI 1388 TGGCCA [CT]^GGCC[GA]
Eco47III 1207 AGCGCT AGC^GCT
EcoRV 337 GATATC GAT^ATC
FauI 791 CCCGC CCCGC(4/6)
FbaI 848 TGATCA T^GATCA
HgaI 1738 GACGC GACGC(5/10)
HindIII 1018 AAGCTT A^AGCTT
HpaI 295 GTTAAC GTT^AAC
KasI 117 GGCGCC G^GCGCC
MluNI 1388 TGGCCA TGG^CCA
MroI 158 TCCGGA T^CCGGA
MscI 1388 TGGCCA TGG^CCA
MslI 679 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 793 CGCG CG^CG
NaeI 1078 GCCGGC GCC^GGC
NarI 117 GGCGCC GG^CGCC
NciI 1455 CCGGG CC^[GC]GG
NcoI 452 CCATGG C^CATGG
NgoMIV 1078 GCCGGC G^CCGGC
PflFI 1686 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1470 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1445 CAGCTG CAG^CTG
RsaI 648 GTAC GT^AC
SexAI 1687 ACCAGGT A^CC[AT]GGT
SfoI 117 GGCGCC GGC^GCC
Tth111I 1686 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1470 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 127 CCAGGAAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 117 GGCGCC G[GA]^CG[CT]C
1738 GACGCC
AvaI 1548 CTCGAG C^[CT]CG[GA]G
1576 CTCGAG
AvaII 1685 GGACC G^G[AT]CC
1691 GGTCC
BbsI 1735 GAAGAC GAAGAC(2/6)
616 GTCTTC
BpuAI 1735 GAAGAC GAAGAC(2/6)
616 GTCTTC
BsaHI 117 GGCGCC G[GA]^CG[CT]C
1738 GACGCC
BsoBI 1548 CTCGAG C^[CT]CG[GA]G
1576 CTCGAG
BstYI 155 AGATCC [GA]^GATC[CT]
1747 GGATCT
DraII 417 GGGGCCC [GA]G^G[ACGT]CC[CT]
418 GGGCCCT
EcoNI 121 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1575 CCTCGAGGAGG
EcoO109I 417 GGGGCCC [GA]G^G[ACGT]CC[CT]
418 GGGCCCT
HaeII 117 GGCGCC [GA]GCGC^[CT]
1207 AGCGCT
Hin1I 117 GGCGCC G[GA]^CG[CT]C
1738 GACGCC
HincII 295 GTTAAC GT[CT]^[GA]AC
1450 GTCAAC
HindII 295 GTTAAC GT[CT]^[GA]AC
1450 GTCAAC
MflI 155 AGATCC [GA]^GATC[CT]
1747 GGATCT
MspA1I 879 CCGCTG C[AC]G^C[GT]G
1445 CAGCTG
PaeR7I 1548 CTCGAG C^TCGAG
1576 CTCGAG
PstI 97 CTGCAG CTGCA^G
898 CTGCAG
SfaNI 1600 GATGC GCATC(5/9)
1082 GCATC
XhoI 1548 CTCGAG C^TCGAG
1576 CTCGAG
XhoII 155 AGATCC [GA]^GATC[CT]
1747 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I