Restriction map for
pFN21AE2514
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# NO CUT ENZYMES ( 82 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI BbvCI BciVI
BfrI BglII BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
HindIII HpaI KpnI MfeI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 2653 GACGTC GACGT^C
AccI 2593 GTCTAC GT^[AC][GT]AC
AcsI 840 GAATTT [GA]^AATT[CT]
ApoI 840 GAATTT [GA]^AATT[CT]
Asp700I 1176 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1036 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 875 TGCGCA TGC^GCA
AvrII 1809 CCTAGG C^CTAGG
BamHI 347 GGATCC G^GATCC
BbeI 1861 GGCGCC GGCGC^C
BbrPI 2388 CACGTG CAC^GTG
BclI 497 TGATCA T^GATCA
BfaI 1810 CTAG C^TAG
BlnI 1809 CCTAGG C^CTAGG
BlpI 1683 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1683 GCTGAGC GC^T[ACGT]AGC
BsaAI 2388 CACGTG [CT]AC^GT[GA]
BsaBI 342 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 570 GGTCTC GGTCTC(1/5)
BsgI 432 GTGCAG GTGCAG(16/14)
BsmAI 571 GTCTC GTCTC(1/5)
BsrDI 420 CATTGC GCAATG(2/0)
BstYI 347 GGATCC [GA]^GATC[CT]
CelII 1683 GCTGAGC GC^T[ACGT]AGC
EcoNI 276 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 497 TGATCA T^GATCA
FspI 875 TGCGCA TGC^GCA
HincII 1951 GTCAAC GT[CT]^[GA]AC
HindII 1951 GTCAAC GT[CT]^[GA]AC
KasI 1861 GGCGCC G^GCGCC
MaeI 1810 CTAG C^TAG
MamI 342 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 347 GGATCC [GA]^GATC[CT]
NarI 1861 GGCGCC GG^CGCC
PflFI 1036 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 966 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2388 CACGTG CAC^GTG
PmlI 2388 CACGTG CAC^GTG
PshAI 97 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2001 GCTCTTC GCTCTTC(1/4)
ScaI 2396 AGTACT AGT^ACT
SfoI 1861 GGCGCC GGC^GCC
StuI 436 AGGCCT AGG^CCT
Tsp509I 841 AATT ^AATT
Tth111I 1036 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 966 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 347 GGATCC [GA]^GATC[CT]
XmnI 1176 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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Alw44I 1330 GTGCAC G^TGCAC
2437 GTGCAC
AlwI 348 GATCC GGATC(4/5)
347 GGATC
AlwNI 59 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2128 CAGTGGCTG
ApaI 885 GGGCCC GGGCC^C
2346 GGGCCC
ApaLI 1330 GTGCAC G^TGCAC
2437 GTGCAC
BalI 174 TGGCCA TGG^CCA
1061 TGGCCA
BcgI 1319 GCAATCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2194 GCACGACAGTCG
BspHI 836 TCATGA T^CATGA
2162 TCATGA
BssSI 1558 CACGAG CACGAG(-5/-1)
1582 CACGAG
BstAPI 668 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1277 GCAAGTCGTGC
BstXI 1505 CCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1751 CCAAGGCGCTGG
EarI 946 CTCTTC CTCTTC(1/4)
2002 CTCTTC
FseI 758 GGCCGGCC GGCCGG^CC
1452 GGCCGGCC
Ksp632I 946 CTCTTC CTCTTC(1/4)
2002 CTCTTC
KspI 781 CCGCGG CCGC^GG
1149 CCGCGG
MluI 1385 ACGCGT A^CGCGT
2492 ACGCGT
MluNI 174 TGGCCA TGG^CCA
1061 TGGCCA
MscI 174 TGGCCA TGG^CCA
1061 TGGCCA
MslI 1021 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2061 CATCAAGGTG
NcoI 1058 CCATGG C^CATGG
1079 CCATGG
NspI 673 ACATGC [GA]CATG^[CT]
1430 ACATGC
PvuII 445 CAGCTG CAG^CTG
1372 CAGCTG
RcaI 836 TCATGA T^CATGA
2162 TCATGA
SacII 781 CCGCGG CCGC^GG
1149 CCGCGG
SfiI 428 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2287 GGCCTCTCGGGCC
SmlI 1408 CTCAAG C^T[CT][GA]AG
1528 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SgrAI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XmaCI XmaI