Restriction map for
pFN21AE1886
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BspDI BspEI BspLU11I
BspMI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tth111I XcmI
XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 794 GGCGCC G[GA]^CG[CT]C
AocI 1006 CCTAAGG CC^T[ACGT]AGG
Aor51HI 548 AGCGCT AGC^GCT
Asp700I 718 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 794 GGCGCC GGCGC^C
BcgI 1114 GCATCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 661 CTAG C^TAG
BsaBI 735 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 794 GGCGCC G[GA]^CG[CT]C
BsmI 671 GCATTC GAATGC(1/-1)
Bsp1407I 1091 TGTACA T^GTACA
BspHI 839 TCATGA T^CATGA
BsrDI 537 CATTGC GCAATG(2/0)
BsrFI 857 ACCGGC [GA]^CCGG[CT]
BsrGI 1091 TGTACA T^GTACA
BsrI 357 ACTGG ACTGG(1/-1)
BssSI 727 CACGAG CACGAG(-5/-1)
Bsu36I 1006 CCTAAGG CC^T[ACGT]AGG
Cfr10I 857 ACCGGC [GA]^CCGG[CT]
DraIII 727 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 548 AGCGCT AGC^GCT
Eco81I 1006 CCTAAGG CC^T[ACGT]AGG
EcoRI 448 GAATTC G^AATTC
EcoRV 709 GATATC GAT^ATC
EcoT22I 247 ATGCAT ATGCA^T
Hin1I 794 GGCGCC G[GA]^CG[CT]C
KasI 794 GGCGCC G^GCGCC
MaeI 661 CTAG C^TAG
MaeII 191 ACGT A^CGT
MamI 735 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 794 GGCGCC GG^CGCC
NcoI 774 CCATGG C^CATGG
NsiI 247 ATGCAT ATGCA^T
PshAI 186 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 839 TCATGA T^CATGA
SacI 505 GAGCTC GAGCT^C
SapI 507 GCTCTTC GCTCTTC(1/4)
SfoI 794 GGCGCC GGC^GCC
SspBI 1091 TGTACA T^GTACA
XbaI 660 TCTAGA T^CTAGA
XmnI 718 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 1092 GTAC GT^AC
1167 GTAC
AvaI 227 CCCGGG C^[CT]CG[GA]G
870 CCCGGG
BanI 465 GGTGCC G^G[CT][GA]CC
794 GGCGCC
BglII 137 AGATCT A^GATCT
786 AGATCT
BpmI 238 CTGGAG CTGGAG(16/14)
1147 CTGGAG
BseRI 241 GAGGAG GAGGAG(10/8)
934 GAGGAG
BsgI 270 CTGCAC GTGCAG(16/14)
1058 GTGCAG
BsoBI 227 CCCGGG C^[CT]CG[GA]G
870 CCCGGG
BstF5I 1161 CATCC GGATG(2/0)
768 GGATG
BstXI 774 CCATGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1205 CCAACATGGTGG
FauI 462 GCGGG CCCGC(4/6)
897 GCGGG
FokI 1161 CATCC GGATG(9/13)
768 GGATG
HaeII 548 AGCGCT [GA]GCGC^[CT]
794 GGCGCC
MaeIII 610 GTCAC ^GT[ACGT]AC
686 GTCAC
MslI 775 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1209 CATGGTGGTG
PflMI 333 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCAGGACCTGG
PleI 289 GAGTC GAGTC(4/5)
646 GAGTC
RsaI 1092 GTAC GT^AC
1167 GTAC
SmaI 227 CCCGGG CCC^GGG
870 CCCGGG
SmlI 141 CTTGAG C^T[CT][GA]AG
799 CTCAAG
TfiI 567 GAATC G^A[AT]TC
846 GATTC
Tsp45I 610 GTCAC ^GT[GC]AC
686 GTCAC
Van91I 333 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCAGGACCTGG
XmaCI 227 CCCGGG C^CCGGG
870 CCCGGG
XmaI 227 CCCGGG C^CCGGG
870 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaII BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp509I TspRI TthHB8I
XhoII