Restriction map for
pFN21AE6261
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvaI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 35 CGCG CG^CG
AfaI 545 GTAC GT^AC
AflIII 519 ACATGT A^C[GA][CT]GT
Asp700I 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 74 GAGCAC G[AT]GC[AT]^C
BcnI 533 CCCGG CC^[GC]GG
BmyI 74 GAGCAC G[AGT]GC[ACT]^C
BpmI 677 CTGGAG CTGGAG(16/14)
BsaJI 532 CCCCGG C^C[ACGT][ACGT]GG
BsiHKAI 74 GAGCAC G[AT]GC[AT]^C
BsiYI 374 CCAGAGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 374 CCAGAGGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 157 GAGAC GTCTC(1/5)
Bsp1286I 74 GAGCAC G[AGT]GC[ACT]^C
BspHI 53 TCATGA T^CATGA
BspLU11I 519 ACATGT A^CATGT
BssKI 533 CCCGG ^CC[ACGT]GG
BstUI 35 CGCG CG^CG
BstXI 416 CCAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 693 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 268 GCGC GCG^C
DdeI 647 CTGAG C^T[ACGT]AG
DraI 66 TTTAAA TTT^AAA
HapII 534 CCGG C^CGG
HhaI 268 GCGC GCG^C
HinP1I 268 GCGC G^CGC
HpaII 534 CCGG C^CGG
HphI 229 GGTGA GGTGA(8/7)
MaeII 543 ACGT A^CGT
MslI 331 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 674 CAGCTG C[AC]G^C[GT]G
MspI 534 CCGG C^CGG
MvnI 35 CGCG CG^CG
NciI 533 CCCGG CC^[GC]GG
NlaIV 690 GGAGCC GG[ACGT]^[ACGT]CC
NruI 34 TCGCGA TCG^CGA
NspI 519 ACATGT [GA]CATG^[CT]
PstI 451 CTGCAG CTGCA^G
PvuII 674 CAGCTG CAG^CTG
RcaI 53 TCATGA T^CATGA
RsaI 545 GTAC GT^AC
ScrFI 533 CCCGG CC^[ACGT]GG
SfaNI 112 GATGC GCATC(5/9)
Tsp45I 230 GTGAC ^GT[GC]AC
TspRI 422 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 22 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 138 GATCC GGATC(4/5)
726 GGATC
BbvI 673 GCAGC GCAGC(8/12)
193 GCTGC
BfaI 205 CTAG C^TAG
442 CTAG
BspMI 226 GCAGGT ACCTGC(4/8)
304 GCAGGT
BstF5I 474 GGATG GGATG(2/0)
573 GGATG
BstYI 137 AGATCC [GA]^GATC[CT]
726 GGATCT
DpnII 138 GATC ^GATC
727 GATC
Fnu4HI 193 GCTGC GC^[ACGT]GC
673 GCAGC
FokI 474 GGATG GGATG(9/13)
573 GGATG
HinfI 279 GATTC G^A[ACGT]TC
630 GATTC
ItaI 193 GCTGC GC^[ACGT]GC
673 GCAGC
MaeI 205 CTAG C^TAG
442 CTAG
MboI 138 GATC ^GATC
727 GATC
MflI 137 AGATCC [GA]^GATC[CT]
726 GGATCT
NdeII 138 GATC ^GATC
727 GATC
Sau3AI 138 GATC ^GATC
727 GATC
TfiI 279 GATTC G^A[AT]TC
630 GATTC
TseI 193 GCTGC G^C[AT]GC
673 GCAGC
XhoII 137 AGATCC [GA]^GATC[CT]
726 GGATCT
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BsrI EarI Ksp632I MaeIII
MboII MnlI MseI NlaIII SfcI
TaqI Tru9I Tsp509I TthHB8I