Restriction map for
pFN21AE3990
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BclI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HpaI KasI KpnI KspI
MaeI MfeI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 154 GTAGAC GT^[AC][GT]AC
AccII 619 CGCG CG^CG
AcyI 468 GGCGTC G[GA]^CG[CT]C
AflIII 618 ACGCGT A^C[GA][CT]GT
AlwI 479 GATCC GGATC(4/5)
AlwNI 540 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 278 AGCGCT AGC^GCT
ApaI 239 GGGCCC GGGCC^C
BanI 361 GGTGCC G^G[CT][GA]CC
BanII 239 GGGCCC G[GA]GC[CT]^C
BcgI 225 GCAGTACTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 173 GGATAC GTATCC(6/5)
BglII 179 AGATCT A^GATCT
BmyI 239 GGGCCC G[AGT]GC[ACT]^C
BsaBI 174 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 468 GGCGTC G[GA]^CG[CT]C
BsgI 587 CTGCAC GTGCAG(16/14)
Bsp1286I 239 GGGCCC G[AGT]GC[ACT]^C
BspHI 296 TCATGA T^CATGA
BstAPI 59 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 619 CGCG CG^CG
BstXI 430 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 279 GCGC GCG^C
EarI 526 GAAGAG CTCTTC(1/4)
Eco47III 278 AGCGCT AGC^GCT
FauI 237 GCGGG CCCGC(4/6)
HaeII 278 AGCGCT [GA]GCGC^[CT]
HhaI 279 GCGC GCG^C
Hin1I 468 GGCGTC G[GA]^CG[CT]C
HinP1I 279 GCGC G^CGC
HincII 127 GTTGAC GT[CT]^[GA]AC
HindII 127 GTTGAC GT[CT]^[GA]AC
HindIII 418 AAGCTT A^AGCTT
Ksp632I 526 GAAGAG CTCTTC(1/4)
MaeII 314 ACGT A^CGT
MaeIII 311 GTGAC ^GT[ACGT]AC
MamI 174 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 618 ACGCGT A^CGCGT
MslI 162 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 543 CAGCTG C[AC]G^C[GT]G
MvnI 619 CGCG CG^CG
PstI 191 CTGCAG CTGCA^G
PvuII 543 CAGCTG CAG^CTG
RcaI 296 TCATGA T^CATGA
ScaI 227 AGTACT AGT^ACT
SfcI 191 CTGCAG C^T[GA][CT]AG
Tsp45I 311 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AluI 419 AGCT AG^CT
544 AGCT
BbsI 141 GAAGAC GAAGAC(2/6)
444 GAAGAC
BpuAI 141 GAAGAC GAAGAC(2/6)
444 GAAGAC
BseRI 102 CTCCTC GAGGAG(10/8)
504 GAGGAG
BsiYI 346 CCGGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
365 CCTCACCGGGG
BslI 346 CCGGGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
365 CCTCACCGGGG
BsmFI 44 GGGAC GGGAC(10/14)
185 GGGAC
BspMI 64 ACCTGC ACCTGC(4/8)
518 GCAGGT
BstF5I 318 GGATG GGATG(2/0)
440 GGATG
BstYI 179 AGATCT [GA]^GATC[CT]
478 AGATCC
FokI 318 GGATG GGATG(9/13)
440 GGATG
HaeIII 240 GGCC GG^CC
389 GGCC
HgaI 469 GCGTC GACGC(5/10)
620 GCGTC
HinfI 46 GACTC G^A[ACGT]TC
609 GAGTC
HphI 83 TCACC GGTGA(8/7)
367 TCACC
MflI 179 AGATCT [GA]^GATC[CT]
478 AGATCC
MseI 134 TTAA T^TAA
604 TTAA
NlaIII 297 CATG CATG^
563 CATG
NlaIV 239 GGGCCC GG[ACGT]^[ACGT]CC
361 GGTGCC
PleI 46 GACTC GAGTC(4/5)
609 GAGTC
SfaNI 331 GATGC GCATC(5/9)
161 GCATC
Tru9I 134 TTAA T^TAA
604 TTAA
XhoII 179 AGATCT [GA]^GATC[CT]
478 AGATCC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AfaI BbvI BcnI BsaJI
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI HapII HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII RsaI Sau3AI
Sau96I ScrFI TseI TspRI