Restriction map for
pFN21AE1896
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MluI MluNI MroI MscI MslI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AflII 373 CTTAAG C^TTAAG
Alw44I 860 GTGCAC G^TGCAC
AlwNI 1028 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 860 GTGCAC G^TGCAC
AspHI 860 GTGCAC G[AT]GC[AT]^C
BanII 217 GAGCCC G[GA]GC[CT]^C
BbvCI 331 GCTGAGG CCTCAGC(-5/-2)
BfrI 373 CTTAAG C^TTAAG
BpmI 614 CTGGAG CTGGAG(16/14)
BsaBI 337 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 860 GTGCAC G[AT]GC[AT]^C
BsmFI 1072 GTCCC GGGAC(10/14)
BstAPI 322 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 540 GGATCT [GA]^GATC[CT]
DraI 949 TTTAAA TTT^AAA
DraII 229 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 229 GGGGCCT [GA]G^G[ACGT]CC[CT]
HincII 531 GTCAAC GT[CT]^[GA]AC
HindII 531 GTCAAC GT[CT]^[GA]AC
MaeII 249 ACGT A^CGT
MamI 337 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1010 CAATTG C^AATTG
MflI 540 GGATCT [GA]^GATC[CT]
MunI 1010 CAATTG C^AATTG
PstI 447 CTGCAG CTGCA^G
ScaI 780 AGTACT AGT^ACT
SmlI 373 CTTAAG C^T[CT][GA]AG
XbaI 367 TCTAGA T^CTAGA
XhoII 540 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 540 GGATC GGATC(4/5)
962 GGATC
BbsI 436 GAAGAC GAAGAC(2/6)
279 GTCTTC
BclI 207 TGATCA T^GATCA
510 TGATCA
BmyI 217 GAGCCC G[AGT]GC[ACT]^C
860 GTGCAC
BpuAI 436 GAAGAC GAAGAC(2/6)
279 GTCTTC
BsmI 1203 GAATGC GAATGC(1/-1)
657 GCATTC
Bsp1286I 217 GAGCCC G[AGT]GC[ACT]^C
860 GTGCAC
BspMI 318 ACCTGC ACCTGC(4/8)
804 ACCTGC
BsrI 778 CCAGT ACTGG(1/-1)
839 CCAGT
FbaI 207 TGATCA T^GATCA
510 TGATCA
HphI 307 GGTGA GGTGA(8/7)
571 GGTGA
MspA1I 1094 CAGCTG C[AC]G^C[GT]G
1228 CAGCTG
NlaIII 25 CATG CATG^
99 CATG
NlaIV 229 GGGGCC GG[ACGT]^[ACGT]CC
1005 GGAGCC
PleI 439 GACTC GAGTC(4/5)
277 GAGTC
PvuII 1094 CAGCTG CAG^CTG
1228 CAGCTG
SfcI 447 CTGCAG C^T[GA][CT]AG
1276 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI