Restriction map for
pFN21AE0602
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AvaI AviII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BpmI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KspI MluI MroI MslI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 504 CCGC CCGC(-3/-1)
AflIII 832 ACATGT A^C[GA][CT]GT
AspI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 670 CCTAGG C^CTAGG
BamHI 40 GGATCC G^GATCC
BanII 787 GGGCTC G[GA]GC[CT]^C
BcnI 546 CCCGG CC^[GC]GG
BfaI 671 CTAG C^TAG
BglI 600 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 670 CCTAGG C^CTAGG
BlpI 580 GCTGAGC GC^T[ACGT]AGC
BmyI 787 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 580 GCTGAGC GC^T[ACGT]AGC
BsaAI 484 TACGTG [CT]AC^GT[GA]
BsaBI 622 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 377 GAGACG CGTCTC(1/5)
BsmI 161 GAATGC GAATGC(1/-1)
Bsp1286I 787 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 832 ACATGT A^CATGT
BsrBI 504 CCGCTC CCGCTC(-3/-3)
BssSI 307 CTCGTG CACGAG(-5/-1)
BstAPI 327 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 984 CCAGGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 336 GCAAGC GC[ACGT]^[ACGT]GC
CelII 580 GCTGAGC GC^T[ACGT]AGC
DraIII 232 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 312 GAAGAG CTCTTC(1/4)
EcoNI 978 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 547 CCGG C^CGG
HincII 193 GTTAAC GT[CT]^[GA]AC
HindII 193 GTTAAC GT[CT]^[GA]AC
HindIII 424 AAGCTT A^AGCTT
HpaI 193 GTTAAC GTT^AAC
HpaII 547 CCGG C^CGG
Ksp632I 312 GAAGAG CTCTTC(1/4)
MaeI 671 CTAG C^TAG
MamI 622 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 547 CCGG C^CGG
NciI 546 CCCGG CC^[GC]GG
NspI 832 ACATGT [GA]CATG^[CT]
PflFI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 267 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 76 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 312 GAAGAGC GCTCTTC(1/4)
SspI 245 AATATT AAT^ATT
Tth111I 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 267 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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Acc65I 294 GGTACC G^GTACC
500 GGTACC
AlwNI 328 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
725 CAGACCCTG
Asp718I 294 GGTACC G^GTACC
500 GGTACC
AvaII 460 GGACC G^G[AT]CC
813 GGACC
BalI 92 TGGCCA TGG^CCA
607 TGGCCA
BsmAI 377 GAGAC GTCTC(1/5)
803 GTCTC
BsmFI 74 GGGAC GGGAC(10/14)
527 GGGAC
BsrI 457 ACTGG ACTGG(1/-1)
991 ACTGG
BstYI 40 GGATCC [GA]^GATC[CT]
660 GGATCT
EaeI 92 TGGCCA [CT]^GGCC[GA]
607 TGGCCA
EcoT14I 653 CCTTGG C^C[AT][AT]GG
670 CCTAGG
HinfI 112 GAATC G^A[ACGT]TC
696 GAATC
HphI 231 TCACC GGTGA(8/7)
852 TCACC
KpnI 294 GGTACC GGTAC^C
500 GGTACC
MaeII 380 ACGT A^CGT
485 ACGT
MfeI 363 CAATTG C^AATTG
563 CAATTG
MflI 40 GGATCC [GA]^GATC[CT]
660 GGATCT
MluNI 92 TGGCCA TGG^CCA
607 TGGCCA
MscI 92 TGGCCA TGG^CCA
607 TGGCCA
MspA1I 631 CAGCTG C[AC]G^C[GT]G
841 CAGCTG
MunI 363 CAATTG C^AATTG
563 CAATTG
PvuII 631 CAGCTG CAG^CTG
841 CAGCTG
ScaI 701 AGTACT AGT^ACT
1028 AGTACT
SexAI 233 ACCTGGT A^CC[AT]GGT
493 ACCAGGT
SmlI 166 CTCAAG C^T[CT][GA]AG
175 CTCAAG
StyI 653 CCTTGG C^C[AT][AT]GG
670 CCTAGG
TfiI 112 GAATC G^A[AT]TC
696 GAATC
XhoII 40 GGATCC [GA]^GATC[CT]
660 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BanI BbsI BbvI BpuAI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII ItaI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI
Tsp45I Tsp509I TspRI