Restriction map for
pFN21AB9602
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaI BsaWI BseAI BsiHKAI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MfeI MluI MroI MunI NcoI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflIII 788 ACGTGT A^C[GA][CT]GT
ApaI 291 GGGCCC GGGCC^C
Asp700I 636 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 575 TGGCCA TGG^CCA
BbsI 1443 GTCTTC GAAGAC(2/6)
BfaI 1150 CTAG C^TAG
BpuAI 1443 GTCTTC GAAGAC(2/6)
BsaBI 573 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 946 CTGCAC GTGCAG(16/14)
BsiWI 1118 CGTACG C^GTACG
BspMI 1223 ACCTGC ACCTGC(4/8)
BsrBI 825 CCGCTC CCGCTC(-3/-3)
BssHII 868 GCGCGC G^CGCGC
BstAPI 300 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1112 AGATCC [GA]^GATC[CT]
MaeI 1150 CTAG C^TAG
MamI 573 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1112 AGATCC [GA]^GATC[CT]
MluNI 575 TGGCCA TGG^CCA
MscI 575 TGGCCA TGG^CCA
MspA1I 301 CAGCTG C[AC]G^C[GT]G
NheI 1149 GCTAGC G^CTAGC
PpuMI 1192 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 301 CAGCTG CAG^CTG
SfcI 1020 CTACAG C^T[GA][CT]AG
SfiI 843 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 444 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 744 CCCGGG CCC^GGG
XhoII 1112 AGATCC [GA]^GATC[CT]
XmaCI 744 CCCGGG C^CCGGG
XmaI 744 CCCGGG C^CCGGG
XmnI 636 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 534 GTAC GT^AC
1119 GTAC
AlwI 36 GATCC GGATC(4/5)
1113 GATCC
AlwNI 298 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
301 CAGCTGCTG
BanII 291 GGGCCC G[GA]GC[CT]^C
588 GAGCCC
BglI 414 GCCCGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
844 GCCAAGCTGGC
BseRI 1320 CTCCTC GAGGAG(10/8)
460 GAGGAG
BspHI 569 TCATGA T^CATGA
632 TCATGA
DdeI 1037 CTCAG C^T[ACGT]AG
1084 CTGAG
DpnII 36 GATC ^GATC
1113 GATC
DrdI 1093 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1392 GACGGGACTGTC
DsaI 108 CCACGG C^C[GA][CT]GG
806 CCACGG
HgaI 258 GACGC GACGC(5/10)
1315 GCGTC
HinfI 1035 GACTC G^A[ACGT]TC
1360 GAGTC
MaeIII 977 GTTAC ^GT[ACGT]AC
1244 GTTAC
MboI 36 GATC ^GATC
1113 GATC
MseI 1366 TTAA T^TAA
1459 TTAA
NaeI 445 GCCGGC GCC^GGC
907 GCCGGC
NdeII 36 GATC ^GATC
1113 GATC
NgoMIV 445 GCCGGC G^CCGGC
907 GCCGGC
PleI 1035 GACTC GAGTC(4/5)
1360 GAGTC
RcaI 569 TCATGA T^CATGA
632 TCATGA
RsaI 534 GTAC GT^AC
1119 GTAC
Sau3AI 36 GATC ^GATC
1113 GATC
TaqI 778 TCGA T^CGA
1345 TCGA
Tru9I 1366 TTAA T^TAA
1459 TTAA
TthHB8I 778 TCGA T^CGA
1345 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HpaII HphI ItaI
KasI MaeII MboII MnlI MslI
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV Sau96I ScrFI
SfaNI SfoI TseI