##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        MvnI        
NaeI        NarI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AspHI         162  GAGCAC               G[AT]GC[AT]^C
AvaI          377  CCCGGG               C^[CT]CG[GA]G
BanI          448  GGCACC               G^G[CT][GA]CC
BbsI          415  GTCTTC               GAAGAC(2/6)
BbvCI         153  GCTGAGG              CCTCAGC(-5/-2)
BglI          189  GCCTGCTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         415  GTCTTC               GAAGAC(2/6)
BseRI         145  GAGGAG               GAGGAG(10/8)
BsiHKAI       162  GAGCAC               G[AT]GC[AT]^C
BsmAI         287  GAGAC                GTCTC(1/5)
BsmI           64  GCATTC               GAATGC(1/-1)
BsoBI         377  CCCGGG               C^[CT]CG[GA]G
BsrDI          73  GCAATG               GCAATG(2/0)
BsrFI         296  ACCGGC               [GA]^CCGG[CT]
CfoI          474  GCGC                 GCG^C
Cfr10I        296  ACCGGC               [GA]^CCGG[CT]
DraII         308  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          435  CCATGG               C^C[GA][CT]GG
EaeI          427  CGGCCA               [CT]^GGCC[GA]
EcoO109I      308  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       435  CCATGG               C^C[AT][AT]GG
FauI          336  CCCGC                CCCGC(4/6)
HgaI          344  GCGTC                GACGC(5/10)
HhaI          474  GCGC                 GCG^C
HinP1I        474  GCGC                 G^CGC
HinfI         214  GAGTC                G^A[ACGT]TC
HphI           22  GGTGA                GGTGA(8/7)
MspA1I        111  CAGCTG               C[AC]G^C[GT]G
NcoI          435  CCATGG               C^CATGG
PflMI         430  CCATGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          214  GAGTC                GAGTC(4/5)
PpuMI         308  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         111  CAGCTG               CAG^CTG
SmaI          377  CCCGGG               CCC^GGG
StyI          435  CCATGG               C^C[AT][AT]GG
Van91I        430  CCATGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         377  CCCGGG               C^CCGGG
XmaI          377  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI          315  GTAC                 GT^AC
              483  GTAC                
BcnI          377  CCCGG                CC^[GC]GG
              378  CCGGG               
BfaI          184  CTAG                 C^TAG
              194  CTAG                
BpmI          267  CTGGAG               CTGGAG(16/14)
              469  CTGGAG              
BsrI          104  ACTGG                ACTGG(1/-1)
              227  CCAGT               
HapII         297  CCGG                 C^CGG
              378  CCGG                
HpaII         297  CCGG                 C^CGG
              378  CCGG                
MaeI          184  CTAG                 C^TAG
              194  CTAG                
MboII         232  GAAGA                GAAGA(8/7)
              416  TCTTC               
MspI          297  CCGG                 C^CGG
              378  CCGG                
NciI          377  CCCGG                CC^[GC]GG
              378  CCGGG               
RsaI          315  GTAC                 GT^AC
              483  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BbvI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
EcoRII      Fnu4HI      HaeIII      ItaI        MnlI        
MvaI        MwoI        NlaIII      NlaIV       Sau96I      
ScrFI       SfcI        TseI        TspRI