Restriction map for
pFN21AE5555
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII AccI AccIII AcyI AflII
AflIII AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI AscI Asp700I
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BseAI BseRI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI Hin1I HincII HindII
HindIII HpaI KasI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PshAI PvuI
PvuII RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 865 GGTACC G^GTACC
AclI 10 AACGTT AA^CGTT
AgeI 480 ACCGGT A^CCGGT
Aor51HI 1009 AGCGCT AGC^GCT
AseI 241 ATTAAT AT^TAAT
AsnI 241 ATTAAT AT^TAAT
Asp718I 865 GGTACC G^GTACC
AspHI 818 GTGCTC G[AT]GC[AT]^C
AviII 738 TGCGCA TGC^GCA
BanI 865 GGTACC G^G[CT][GA]CC
BanII 495 GGGCTC G[GA]GC[CT]^C
BcgI 614 GCACCATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 769 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 580 GAGACC GGTCTC(1/5)
BsaWI 480 ACCGGT [AT]^CCGG[AT]
BsgI 710 CTGCAC GTGCAG(16/14)
BsiEI 785 CGGCCG CG[GA][CT]^CG
BsiHKAI 818 GTGCTC G[AT]GC[AT]^C
BsmI 97 GAATGC GAATGC(1/-1)
BsrFI 480 ACCGGT [GA]^CCGG[CT]
BstAPI 283 GCAATGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 794 CCACAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 480 ACCGGT [GA]^CCGG[CT]
DpnII 621 GATC ^GATC
DraII 354 AGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 785 CGGCCG C^GGCCG
EclXI 785 CGGCCG C^GGCCG
Eco47III 1009 AGCGCT AGC^GCT
Eco52I 785 CGGCCG C^GGCCG
EcoO109I 354 AGGTCCC [GA]G^G[ACGT]CC[CT]
FspI 738 TGCGCA TGC^GCA
HaeII 1009 AGCGCT [GA]GCGC^[CT]
HgaI 890 GCGTC GACGC(5/10)
KpnI 865 GGTACC GGTAC^C
MboI 621 GATC ^GATC
MslI 795 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 621 GATC ^GATC
NotI 784 GCGGCCGC GC^GGCCGC
NspI 76 ACATGC [GA]CATG^[CT]
PinAI 480 ACCGGT A^CCGGT
PpuMI 354 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 241 ATTAAT AT^TAAT
Psp1406I 10 AACGTT AA^CGTT
SapI 820 GCTCTTC GCTCTTC(1/4)
Sau3AI 621 GATC ^GATC
SspI 695 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 103 AAATTT [GA]^AATT[CT]
440 AAATTC
AfaI 866 GTAC GT^AC
994 GTAC
ApoI 103 AAATTT [GA]^AATT[CT]
440 AAATTC
AvaI 492 CCCGGG C^[CT]CG[GA]G
572 CCCGGG
AvaII 355 GGTCC G^G[AT]CC
429 GGTCC
BfaI 235 CTAG C^TAG
400 CTAG
BpmI 416 CTGGAG CTGGAG(16/14)
535 CTGGAG
BsoBI 492 CCCGGG C^[CT]CG[GA]G
572 CCCGGG
BsrDI 283 GCAATG GCAATG(2/0)
860 GCAATG
DdeI 509 CTCAG C^T[ACGT]AG
520 CTCAG
DsaI 782 CCGCGG C^C[GA][CT]GG
788 CCGCGG
EarI 821 CTCTTC CTCTTC(1/4)
143 GAAGAG
HinfI 163 GATTC G^A[ACGT]TC
261 GATTC
Ksp632I 821 CTCTTC CTCTTC(1/4)
143 GAAGAG
KspI 782 CCGCGG CCGC^GG
788 CCGCGG
MaeI 235 CTAG C^TAG
400 CTAG
MaeII 11 ACGT A^CGT
1015 ACGT
MspA1I 782 CCGCGG C[AC]G^C[GT]G
788 CCGCGG
RsaI 866 GTAC GT^AC
994 GTAC
SacII 782 CCGCGG CCGC^GG
788 CCGCGG
SfaNI 300 GATGC GCATC(5/9)
1002 GATGC
SmaI 492 CCCGGG CCC^GGG
572 CCCGGG
TaqI 184 TCGA T^CGA
623 TCGA
TfiI 163 GATTC G^A[AT]TC
261 GATTC
Tsp45I 484 GTCAC ^GT[GC]AC
632 GTGAC
TthHB8I 184 TCGA T^CGA
623 TCGA
XmaCI 492 CCCGGG C^CCGGG
572 CCCGGG
XmaI 492 CCCGGG C^CCGGG
572 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PstI Sau96I
ScrFI SfcI Tru9I TseI Tsp509I
TspRI