Restriction map for
pFN21AE3072
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI AluI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AvaII AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KspI MaeII MluI
MluNI MroI MscI MspI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 132 GGTACC G^GTACC
AccI 71 GTCTAC GT^[AC][GT]AC
AccII 643 CGCG CG^CG
AcsI 361 AAATTC [GA]^AATT[CT]
AfaI 133 GTAC GT^AC
ApoI 361 AAATTC [GA]^AATT[CT]
Asp700I 357 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 132 GGTACC G^GTACC
AvaI 37 CCCGAG C^[CT]CG[GA]G
BamHI 111 GGATCC G^GATCC
BanII 387 GAGCCC G[GA]GC[CT]^C
BfaI 587 CTAG C^TAG
BglII 498 AGATCT A^GATCT
BsaBI 529 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 445 GAGACC GGTCTC(1/5)
BsgI 149 GTGCAG GTGCAG(16/14)
BsiYI 123 CCTTAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 123 CCTTAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 445 GAGAC GTCTC(1/5)
BsmFI 228 GGGAC GGGAC(10/14)
BsoBI 37 CCCGAG C^[CT]CG[GA]G
BsrDI 275 GCAATG GCAATG(2/0)
BstEII 42 GGTCACC G^GT[ACGT]ACC
BstPI 42 GGTCACC G^GT[ACGT]ACC
BstUI 643 CGCG CG^CG
BstXI 404 CCAGATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 14 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 642 GCGC GCG^C
DsaI 576 CCATGG C^C[GA][CT]GG
EarI 202 GAAGAG CTCTTC(1/4)
EcoO65I 42 GGTCACC G^GT[ACGT]ACC
EcoT14I 576 CCATGG C^C[AT][AT]GG
HaeIII 30 GGCC GG^CC
HhaI 642 GCGC GCG^C
HinP1I 642 GCGC G^CGC
HincII 8 GTCAAC GT[CT]^[GA]AC
HindII 8 GTCAAC GT[CT]^[GA]AC
KpnI 132 GGTACC GGTAC^C
Ksp632I 202 GAAGAG CTCTTC(1/4)
MaeI 587 CTAG C^TAG
MamI 529 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 290 CAATTG C^AATTG
MspA1I 22 CCGCTG C[AC]G^C[GT]G
MunI 290 CAATTG C^AATTG
MvnI 643 CGCG CG^CG
MwoI 676 GCTCTTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 576 CCATGG C^CATGG
PstI 683 CTGCAG CTGCA^G
RsaI 133 GTAC GT^AC
SfcI 683 CTGCAG C^T[GA][CT]AG
SpeI 586 ACTAGT A^CTAGT
StyI 576 CCATGG C^C[AT][AT]GG
Tsp45I 43 GTCAC ^GT[GC]AC
TspRI 450 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 357 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 22 CCGC CCGC(-3/-1)
555 GCGG
BanI 132 GGTACC G^G[CT][GA]CC
474 GGCACC
BbvI 685 GCAGC GCAGC(8/12)
18 GCTGC
BmyI 387 GAGCCC G[AGT]GC[ACT]^C
452 GTGCCC
BsaJI 38 CCGAGG C^C[ACGT][ACGT]GG
576 CCATGG
Bsp1286I 387 GAGCCC G[AGT]GC[ACT]^C
452 GTGCCC
BspHI 257 TCATGA T^CATGA
441 TCATGA
BssKI 366 CCTGG ^CC[ACGT]GG
411 CCTGG
BstNI 366 CCTGG CC^[AT]GG
411 CCTGG
BstYI 111 GGATCC [GA]^GATC[CT]
498 AGATCT
EcoRII 366 CCTGG ^CC[AT]GG
411 CCTGG
HphI 580 GGTGA GGTGA(8/7)
44 TCACC
MflI 111 GGATCC [GA]^GATC[CT]
498 AGATCT
MslI 523 CATGTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
535 CATCTCTATG
MvaI 366 CCTGG CC^[AT]GG
411 CCTGG
RcaI 257 TCATGA T^CATGA
441 TCATGA
ScrFI 366 CCTGG CC^[ACGT]GG
411 CCTGG
TseI 18 GCTGC G^C[AT]GC
685 GCAGC
XhoII 111 GGATCC [GA]^GATC[CT]
498 AGATCT
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AlwI BpmI BsrI BstF5I DdeI
DpnII Fnu4HI FokI HinfI ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV Sau3AI SfaNI
TfiI Tru9I Tsp509I