Restriction map for
pFN21AE5530
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 5 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 339 ATTAAT AT^TAAT
AsnI 339 ATTAAT AT^TAAT
BclI 357 TGATCA T^GATCA
BfaI 1141 CTAG C^TAG
BglII 1188 AGATCT A^GATCT
BseRI 818 GAGGAG GAGGAG(10/8)
BsgI 842 GTGCAG GTGCAG(16/14)
BsmFI 242 GTCCC GGGAC(10/14)
BsrDI 197 GCAATG GCAATG(2/0)
BsrI 874 CCAGT ACTGG(1/-1)
BssKI 323 CCAGG ^CC[ACGT]GG
BstNI 323 CCAGG CC^[AT]GG
BstXI 662 CCAAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 513 GCCAGC GC[ACGT]^[ACGT]GC
DraI 427 TTTAAA TTT^AAA
DraII 1014 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 1006 CCTTCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1014 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 323 CCAGG ^CC[AT]GG
EcoRV 600 GATATC GAT^ATC
FbaI 357 TGATCA T^GATCA
HindIII 347 AAGCTT A^AGCTT
HphI 1096 TCACC GGTGA(8/7)
MaeI 1141 CTAG C^TAG
MaeII 449 ACGT A^CGT
MslI 748 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 323 CCAGG CC^[AT]GG
NlaIV 780 GGAGCC GG[ACGT]^[ACGT]CC
PpuMI 1014 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 339 ATTAAT AT^TAAT
ScrFI 323 CCAGG CC^[ACGT]GG
SmlI 461 CTCAAG C^T[CT][GA]AG
SspI 1168 AATATT AAT^ATT
TaqI 1112 TCGA T^CGA
TthHB8I 1112 TCGA T^CGA
XbaI 1140 TCTAGA T^CTAGA
XcmI 1233 CCATGTCCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AccI 264 GTATAC GT^[AC][GT]AC
851 GTATAC
BsiYI 323 CCAGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1006 CCTTCAGTAGG
BslI 323 CCAGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1006 CCTTCAGTAGG
Bsp1407I 1080 TGTACA T^GTACA
1201 TGTACA
BsrGI 1080 TGTACA T^GTACA
1201 TGTACA
Bst1107I 264 GTATAC GTA^TAC
851 GTATAC
BstZ17I 264 GTATAC GTA^TAC
851 GTATAC
EcoRI 476 GAATTC G^AATTC
1154 GAATTC
HaeIII 114 GGCC GG^CC
1246 GGCC
NspI 886 GCATGT [GA]CATG^[CT]
1075 GCATGT
PstI 704 CTGCAG CTGCA^G
972 CTGCAG
SspBI 1080 TGTACA T^GTACA
1201 TGTACA
Tsp45I 406 GTGAC ^GT[GC]AC
823 GTCAC
TspRI 518 CACTG CA[GC]TG[ACGT][ACGT]^
944 CAGTG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbsI BbvI BpuAI BsaJI
BsmI BstF5I BstYI Cfr13I DdeI
DpnII EarI EcoT14I Fnu4HI FokI
HinfI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII PleI RsaI Sau3AI
Sau96I SfcI StyI TfiI Tru9I
TseI Tsp509I XhoII