Restriction map for
pFN21AE2777
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI FauI FseI FspI
HapII HindIII HpaII KasI KspI
MamI MluI MluNI MroI MscI
MspI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 700 GGTACC G^GTACC
AccII 1465 CGCG CG^CG
AciI 1464 CCGC CCGC(-3/-1)
AcyI 352 GACGCC G[GA]^CG[CT]C
AlwNI 2267 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 162 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 700 GGTACC G^GTACC
BamHI 388 GGATCC G^GATCC
BanII 1567 GAGCTC G[GA]GC[CT]^C
BbvCI 1536 CCTCAGC CCTCAGC(-5/-2)
BglII 80 AGATCT A^GATCT
BsaHI 352 GACGCC G[GA]^CG[CT]C
BsmI 486 GAATGC GAATGC(1/-1)
BspHI 1841 TCATGA T^CATGA
BstUI 1465 CGCG CG^CG
DraI 1752 TTTAAA TTT^AAA
DraIII 848 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2128 GAATTC G^AATTC
EcoRV 442 GATATC GAT^ATC
Hin1I 352 GACGCC G[GA]^CG[CT]C
HpaI 46 GTTAAC GTT^AAC
KpnI 700 GGTACC GGTAC^C
MaeII 467 ACGT A^CGT
MfeI 983 CAATTG C^AATTG
MunI 983 CAATTG C^AATTG
MvnI 1465 CGCG CG^CG
RcaI 1841 TCATGA T^CATGA
SacI 1567 GAGCTC GAGCT^C
SapI 1672 GCTCTTC GCTCTTC(1/4)
SexAI 1734 ACCTGGT A^CC[AT]GGT
SpeI 1633 ACTAGT A^CTAGT
XbaI 2476 TCTAGA T^CTAGA
XcmI 591 CCATTATCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 162 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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Aor51HI 341 AGCGCT AGC^GCT
1520 AGCGCT
AspHI 623 GTGCTC G[AT]GC[AT]^C
1567 GAGCTC
BclI 1083 TGATCA T^GATCA
1911 TGATCA
BsiHKAI 623 GTGCTC G[AT]GC[AT]^C
1567 GAGCTC
BsmBI 794 GAGACG CGTCTC(1/5)
964 GAGACG
BssSI 848 CACGAG CACGAG(-5/-1)
2246 CACGAG
BstEII 2349 GGTCACC G^GT[ACGT]ACC
2358 GGTCACC
BstPI 2349 GGTCACC G^GT[ACGT]ACC
2358 GGTCACC
BstXI 297 CCATGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2024 CCAAAGTTGTGG
DsaI 636 CCATGG C^C[GA][CT]GG
2172 CCATGG
Eco47III 341 AGCGCT AGC^GCT
1520 AGCGCT
EcoO65I 2349 GGTCACC G^GT[ACGT]ACC
2358 GGTCACC
EcoT22I 1232 ATGCAT ATGCA^T
1413 ATGCAT
FbaI 1083 TGATCA T^GATCA
1911 TGATCA
HgaI 352 GACGC GACGC(5/10)
966 GACGC
HincII 46 GTTAAC GT[CT]^[GA]AC
522 GTTGAC
HindII 46 GTTAAC GT[CT]^[GA]AC
522 GTTGAC
MslI 298 CATGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2433 CACACCCGTG
MspA1I 755 CAGCTG C[AC]G^C[GT]G
1391 CAGCTG
MwoI 308 GCACTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
886 GCTTCCGAAGC
NcoI 636 CCATGG C^CATGG
2172 CCATGG
NsiI 1232 ATGCAT ATGCA^T
1413 ATGCAT
PleI 937 GAGTC GAGTC(4/5)
1373 GAGTC
PstI 1285 CTGCAG CTGCA^G
1553 CTGCAG
PvuII 755 CAGCTG CAG^CTG
1391 CAGCTG
SfcI 1285 CTGCAG C^T[GA][CT]AG
1553 CTGCAG
TaqI 377 TCGA T^CGA
627 TCGA
TspRI 612 CACTG CA[GC]TG[ACGT][ACGT]^
928 CAGTG
TthHB8I 377 TCGA T^CGA
627 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI AvaII BanI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV PpuMI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp45I Tsp509I XhoII