Restriction map for
pFN21AB5871
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AspHI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BclI BcnI BglI
BglII BlnI BpmI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 593 GGTACC G^GTACC
AcsI 1069 GAATTT [GA]^AATT[CT]
AflII 217 CTTAAG C^TTAAG
AlwNI 937 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1235 CCTCAGG CC^T[ACGT]AGG
ApoI 1069 GAATTT [GA]^AATT[CT]
AseI 834 ATTAAT AT^TAAT
AsnI 834 ATTAAT AT^TAAT
Asp718I 593 GGTACC G^GTACC
AvaI 371 CTCGGG C^[CT]CG[GA]G
BalI 1155 TGGCCA TGG^CCA
BamHI 570 GGATCC G^GATCC
BanI 593 GGTACC G^G[CT][GA]CC
BanII 599 GAGCCC G[GA]GC[CT]^C
BbsI 1009 GAAGAC GAAGAC(2/6)
BciVI 1336 GGATAC GTATCC(6/5)
BfrI 217 CTTAAG C^TTAAG
BlpI 24 GCTGAGC GC^T[ACGT]AGC
BmyI 599 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
BpuAI 1009 GAAGAC GAAGAC(2/6)
BsaAI 858 TACGTA [CT]AC^GT[GA]
BsaBI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 1191 GGGAC GGGAC(10/14)
BsmI 922 GCATTC GAATGC(1/-1)
BsoBI 371 CTCGGG C^[CT]CG[GA]G
Bsp1286I 599 GAGCCC G[AGT]GC[ACT]^C
BspHI 1250 TCATGA T^CATGA
BsrFI 1039 GCCGGT [GA]^CCGG[CT]
Bst1107I 861 GTATAC GTA^TAC
BstXI 1140 CCACACTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 861 GTATAC GTA^TAC
Bsu36I 1235 CCTCAGG CC^T[ACGT]AGG
CelII 24 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1039 GCCGGT [GA]^CCGG[CT]
DsaI 950 CCATGG C^C[GA][CT]GG
Eco81I 1235 CCTCAGG CC^T[ACGT]AGG
EcoNI 490 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 950 CCATGG C^C[AT][AT]GG
HapII 1040 CCGG C^CGG
HincII 707 GTCGAC GT[CT]^[GA]AC
HindII 707 GTCGAC GT[CT]^[GA]AC
HinfI 1167 GAATC G^A[ACGT]TC
HpaII 1040 CCGG C^CGG
KpnI 593 GGTACC GGTAC^C
MamI 565 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1155 TGGCCA TGG^CCA
MscI 1155 TGGCCA TGG^CCA
MslI 1141 CACACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 751 CAGCTG C[AC]G^C[GT]G
MspI 1040 CCGG C^CGG
NcoI 950 CCATGG C^CATGG
PshAI 1174 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 834 ATTAAT AT^TAAT
PvuII 751 CAGCTG CAG^CTG
RcaI 1250 TCATGA T^CATGA
SalI 707 GTCGAC G^TCGAC
ScaI 1367 AGTACT AGT^ACT
SmlI 217 CTTAAG C^T[CT][GA]AG
SnaBI 858 TACGTA TAC^GTA
SpeI 727 ACTAGT A^CTAGT
SphI 426 GCATGC GCATG^C
StyI 950 CCATGG C^C[AT][AT]GG
TfiI 1167 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 607 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
840 GAAAGATTTC
BfaI 406 CTAG C^TAG
728 CTAG
BsiYI 490 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1294 CCCAACCCTGG
BslI 490 CCTGAGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1294 CCCAACCCTGG
BsmAI 1103 GAGAC GTCTC(1/5)
258 GTCTC
BspMI 422 ACCTGC ACCTGC(4/8)
459 ACCTGC
BsrBI 34 GAGCGG CCGCTC(-3/-3)
40 GAGCGG
BssKI 1162 CCAGG ^CC[ACGT]GG
1300 CCTGG
BstF5I 223 CATCC GGATG(2/0)
132 GGATG
BstNI 1162 CCAGG CC^[AT]GG
1300 CCTGG
Cac8I 32 GCGAGC GC[ACGT]^[ACGT]GC
426 GCATGC
DraI 13 TTTAAA TTT^AAA
150 TTTAAA
EaeI 81 TGGCCG [CT]^GGCC[GA]
1155 TGGCCA
EarI 239 CTCTTC CTCTTC(1/4)
500 GAAGAG
EcoRII 1162 CCAGG ^CC[AT]GG
1300 CCTGG
FauI 36 GCGGG CCCGC(4/6)
42 GCGGG
FokI 223 CATCC GGATG(9/13)
132 GGATG
HaeIII 82 GGCC GG^CC
1156 GGCC
Ksp632I 239 CTCTTC CTCTTC(1/4)
500 GAAGAG
MaeI 406 CTAG C^TAG
728 CTAG
MvaI 1162 CCAGG CC^[AT]GG
1300 CCTGG
PstI 785 CTGCAG CTGCA^G
870 CTGCAG
ScrFI 1162 CCAGG CC^[ACGT]GG
1300 CCTGG
SfaNI 816 GATGC GCATC(5/9)
222 GCATC
TspRI 575 CAGTG CA[GC]TG[ACGT][ACGT]^
703 CACTG
XmnI 607 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
840 GAAAGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AvaII BbvI BsaJI BsrI BstYI
Cfr13I DdeI DpnII DraII EcoO109I
Fnu4HI HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MwoI NdeII NlaIII NlaIV NspI
PpuMI RsaI Sau3AI Sau96I SfcI
SspI TaqI Tru9I TseI Tsp45I
Tsp509I TthHB8I XhoII