Restriction map for
pFN21AE5133
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BbvI BcgI
BciVI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI MwoI NaeI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
TseI Tsp509I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 400 GCGG CCGC(-3/-1)
AcyI 54 GGCGCC G[GA]^CG[CT]C
AlwNI 234 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 263 CCTCAGG CC^T[ACGT]AGG
AspHI 3 GAGCAC G[AT]GC[AT]^C
AspI 200 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 54 GGCGCC G^G[CT][GA]CC
BbeI 54 GGCGCC GGCGC^C
BclI 303 TGATCA T^GATCA
BcnI 51 CCCGG CC^[GC]GG
BfaI 319 CTAG C^TAG
BmyI 3 GAGCAC G[AGT]GC[ACT]^C
BsaHI 54 GGCGCC G[GA]^CG[CT]C
BsaWI 117 TCCGGT [AT]^CCGG[AT]
BsgI 149 CTGCAC GTGCAG(16/14)
BsiHKAI 3 GAGCAC G[AT]GC[AT]^C
BsiYI 69 CCGGCTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 69 CCGGCTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 63 GGGAC GGGAC(10/14)
Bsp1286I 3 GAGCAC G[AGT]GC[ACT]^C
Bsu36I 263 CCTCAGG CC^T[ACGT]AGG
CfoI 55 GCGC GCG^C
DdeI 264 CTCAG C^T[ACGT]AG
EarI 181 GAAGAG CTCTTC(1/4)
Eco81I 263 CCTCAGG CC^T[ACGT]AGG
FbaI 303 TGATCA T^GATCA
Fnu4HI 400 GCGGC GC^[ACGT]GC
HaeII 54 GGCGCC [GA]GCGC^[CT]
HaeIII 214 GGCC GG^CC
HhaI 55 GCGC GCG^C
Hin1I 54 GGCGCC G[GA]^CG[CT]C
HinP1I 55 GCGC G^CGC
HphI 364 GGTGA GGTGA(8/7)
ItaI 400 GCGGC GC^[ACGT]GC
KasI 54 GGCGCC G^GCGCC
Ksp632I 181 GAAGAG CTCTTC(1/4)
MaeI 319 CTAG C^TAG
MaeII 88 ACGT A^CGT
MaeIII 112 GTCAC ^GT[ACGT]AC
MnlI 263 CCTC CCTC(7/6)
MseI 464 TTAA T^TAA
NarI 54 GGCGCC GG^CGCC
NciI 51 CCCGG CC^[GC]GG
NcoI 58 CCATGG C^CATGG
NheI 318 GCTAGC G^CTAGC
NlaIII 59 CATG CATG^
PflFI 200 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 181 GAAGAGC GCTCTTC(1/4)
SfaNI 269 GCATC GCATC(5/9)
SfoI 54 GGCGCC GGC^GCC
TaqI 179 TCGA T^CGA
Tru9I 464 TTAA T^TAA
Tsp45I 112 GTCAC ^GT[GC]AC
Tth111I 200 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 179 TCGA T^CGA
XcmI 233 CCAGCAACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BstF5I 258 CATCC GGATG(2/0)
165 GGATG
Cac8I 130 GCAGGC GC[ACGT]^[ACGT]GC
318 GCTAGC
DpnII 296 GATC ^GATC
304 GATC
DsaI 8 CCGTGG C^C[GA][CT]GG
58 CCATGG
EcoT14I 58 CCATGG C^C[AT][AT]GG
161 CCTTGG
FokI 258 CATCC GGATG(9/13)
165 GGATG
HinfI 65 GACTC G^A[ACGT]TC
478 GAGTC
MboI 296 GATC ^GATC
304 GATC
MboII 181 GAAGA GAAGA(8/7)
405 GAAGA
NdeII 296 GATC ^GATC
304 GATC
NlaIV 54 GGCGCC GG[ACGT]^[ACGT]CC
212 GGGGCC
PleI 65 GACTC GAGTC(4/5)
478 GAGTC
Sau3AI 296 GATC ^GATC
304 GATC
StyI 58 CCATGG C^C[AT][AT]GG
161 CCTTGG
TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^
152 CACTG
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI AvaII BsaJI BssKI BstNI
Cfr13I EcoRII HapII HpaII MspI
MvaI Sau96I ScrFI