##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AluI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FokI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MflI        MluI        MroI        
MseI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       TseI        TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          133  GAATTT               [GA]^AATT[CT]
AfaI          147  GTAC                 GT^AC
AflIII         35  ACATGT               A^C[GA][CT]GT
AlwNI          79  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          133  GAATTT               [GA]^AATT[CT]
BalI          242  TGGCCA               TGG^CCA
BclI           20  TGATCA               T^GATCA
BpmI           85  CTGGAG               CTGGAG(16/14)
BsaJI         231  CCTGGG               C^C[ACGT][ACGT]GG
BsiEI           6  CGACCG               CG[GA][CT]^CG
BspLU11I       35  ACATGT               A^CATGT
Cfr13I        234  GGGCC                G^G[ACGT]CC
DdeI           77  CTCAG                C^T[ACGT]AG
DpnII          21  GATC                 ^GATC
EaeI          242  TGGCCA               [CT]^GGCC[GA]
EarI           43  GAAGAG               CTCTTC(1/4)
FbaI           20  TGATCA               T^GATCA
Fnu4HI        115  GCGGC                GC^[ACGT]GC
HaeII          81  GGCGCT               [GA]GCGC^[CT]
HinfI          58  GACTC                G^A[ACGT]TC
ItaI          115  GCGGC                GC^[ACGT]GC
Ksp632I        43  GAAGAG               CTCTTC(1/4)
MaeIII        196  GTGAC                ^GT[ACGT]AC
MboI           21  GATC                 ^GATC
MluNI         242  TGGCCA               TGG^CCA
MnlI          177  GAGG                 CCTC(7/6)
MscI          242  TGGCCA               TGG^CCA
MwoI           73  GCTACTCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          21  GATC                 ^GATC
NspI           35  ACATGT               [GA]CATG^[CT]
PleI           58  GACTC                GAGTC(4/5)
RsaI          147  GTAC                 GT^AC
Sau3AI         21  GATC                 ^GATC
Sau96I        234  GGGCC                G^G[ACGT]CC
Tsp45I        196  GTGAC                ^GT[GC]AC
Tsp509I       134  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           31  GCGG                 CCGC(-3/-1)
              115  GCGG                
BsrDI         111  CATTGC               GCAATG(2/0)
              163  CATTGC              
BssKI         158  CCTGG                ^CC[ACGT]GG
              231  CCTGG               
BstNI         158  CCTGG                CC^[AT]GG
              231  CCTGG               
CfoI           71  GCGC                 GCG^C
               82  GCGC                
EcoRII        158  CCTGG                ^CC[AT]GG
              231  CCTGG               
HhaI           71  GCGC                 GCG^C
               82  GCGC                
HinP1I         71  GCGC                 G^CGC
               82  GCGC                
MboII          43  GAAGA                GAAGA(8/7)
              249  TCTTC               
MvaI          158  CCTGG                CC^[AT]GG
              231  CCTGG               
NlaIII         36  CATG                 CATG^
              202  CATG                
ScrFI         158  CCTGG                CC^[ACGT]GG
              231  CCTGG               
SfaNI          48  GATGC                GCATC(5/9)
              167  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

HaeIII