Restriction map for
pFN21AE4158
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsiWI BspDI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI Eam1105I EarI Eco47III
Eco81I EcoO109I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HindIII HpaI
Ksp632I KspI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SwaI TaqI Tth111I TthHB8I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 976 GACGTC GACGT^C
Acc65I 869 GGTACC G^GTACC
AccII 1471 CGCG CG^CG
AccIII 300 TCCGGA T^CCGGA
AclI 1090 AACGTT AA^CGTT
AcsI 814 GAATTT [GA]^AATT[CT]
Alw44I 218 GTGCAC G^TGCAC
ApaLI 218 GTGCAC G^TGCAC
ApoI 814 GAATTT [GA]^AATT[CT]
Asp700I 847 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 869 GGTACC G^GTACC
AvaI 85 CCCGAG C^[CT]CG[GA]G
BamHI 1175 GGATCC G^GATCC
BbeI 63 GGCGCC GGCGC^C
BbrPI 1107 CACGTG CAC^GTG
BbsI 922 GAAGAC GAAGAC(2/6)
BglII 897 AGATCT A^GATCT
BpuAI 922 GAAGAC GAAGAC(2/6)
BsaBI 479 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1233 GGTCTC GGTCTC(1/5)
BseAI 300 TCCGGA T^CCGGA
BsiEI 112 CGGCCG CG[GA][CT]^CG
BsmBI 978 CGTCTC CGTCTC(1/5)
BsoBI 85 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1005 TGTACA T^GTACA
BspEI 300 TCCGGA T^CCGGA
BspMI 908 ACCTGC ACCTGC(4/8)
BsrDI 471 CATTGC GCAATG(2/0)
BsrGI 1005 TGTACA T^GTACA
BstAPI 1344 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1471 CGCG CG^CG
BstXI 1136 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1345 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 112 CGGCCG C^GGCCG
EclXI 112 CGGCCG C^GGCCG
Eco52I 112 CGGCCG C^GGCCG
EcoRV 148 GATATC GAT^ATC
HincII 1114 GTCAAC GT[CT]^[GA]AC
HindII 1114 GTCAAC GT[CT]^[GA]AC
KasI 63 GGCGCC G^GCGCC
KpnI 869 GGTACC GGTAC^C
MamI 479 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 300 TCCGGA T^CCGGA
MslI 457 CACGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1471 CGCG CG^CG
NarI 63 GGCGCC GG^CGCC
NcoI 1544 CCATGG C^CATGG
PleI 925 GACTC GAGTC(4/5)
PmaCI 1107 CACGTG CAC^GTG
PmlI 1107 CACGTG CAC^GTG
Psp1406I 1090 AACGTT AA^CGTT
PvuII 1495 CAGCTG CAG^CTG
SfcI 9 CTACAG C^T[GA][CT]AG
SfoI 63 GGCGCC GGC^GCC
SnaBI 526 TACGTA TAC^GTA
SspBI 1005 TGTACA T^GTACA
SspI 646 AATATT AAT^ATT
StuI 1284 AGGCCT AGG^CCT
XbaI 972 TCTAGA T^CTAGA
XcmI 990 CCAAGTTGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 847 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 218 GTGCAC G[AT]GC[AT]^C
691 GAGCAC
AvaII 662 GGACC G^G[AT]CC
1491 GGACC
BalI 743 TGGCCA TGG^CCA
1547 TGGCCA
BanII 104 GGGCTC G[GA]GC[CT]^C
1509 GAGCCC
BbvCI 720 CCTCAGC CCTCAGC(-5/-2)
1119 CCTCAGC
BpmI 415 CTGGAG CTGGAG(16/14)
893 CTGGAG
BsaAI 526 TACGTA [CT]AC^GT[GA]
1107 CACGTG
BsaWI 300 TCCGGA [AT]^CCGG[AT]
664 ACCGGA
BsgI 1243 GTGCAG GTGCAG(16/14)
1351 GTGCAG
BsiHKAI 218 GTGCAC G[AT]GC[AT]^C
691 GAGCAC
BsmFI 506 GGGAC GGGAC(10/14)
521 GGGAC
BsmI 572 GAATGC GAATGC(1/-1)
450 GCATTC
BspLU11I 368 ACATGT A^CATGT
967 ACATGT
EcoNI 590 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
720 CCTCAGCCAGG
EcoT14I 359 CCAAGG C^C[AT][AT]GG
1544 CCATGG
HaeII 32 GGCGCT [GA]GCGC^[CT]
63 GGCGCC
HgaI 1067 GACGC GACGC(5/10)
1540 GACGC
MluNI 743 TGGCCA TGG^CCA
1547 TGGCCA
MscI 743 TGGCCA TGG^CCA
1547 TGGCCA
MseI 644 TTAA T^TAA
658 TTAA
MspA1I 887 CCGCTG C[AC]G^C[GT]G
1495 CAGCTG
StyI 359 CCAAGG C^C[AT][AT]GG
1544 CCATGG
TfiI 390 GAATC G^A[AT]TC
544 GAATC
Tru9I 644 TTAA T^TAA
658 TTAA
Tsp509I 167 AATT ^AATT
815 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI BanI BbvI BcnI
BfaI BmyI BsaHI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI XhoII